Ann Thorac Surg 1982;33:453-458
© 1982 The Society of Thoracic Surgeons
Articles
Myocardial High-Energy Phosphate Replenishment during Ischemic Arrest: Aerobic versus Anaerobic Metabolism
Richard M. Engelman, M.D.*,
William A. Dobbs, Ph.D.,
John H. Rousou, M.D.,
Mooideen K. Meeran, M.D., Ph.D.
From the Departments of Surgery, the University of Connecticut Health Center, Farmington, CT, and the Baystate Medical Center, Springfield, MA
Accepted for publication July 16, 1981.
* Address reprint requests to Dr. Engelman, Chief, Cardiac Surgery, Baystate Medical Center, 759 Chestnut St, Springfield, MA 01107
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Abstract
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An in vivo, isolated pig heart preparation was used to study the effect of L-glutamate added to crystalloid and blood potassium cardioplegia on the myocardial high-energy phosphate compounds, adenosine triphosphate (ATP) and creatine phosphate (CP). Studies were performed during a three-hour arrest interval and during 60 minutes of reperfusion. Levels of ATP remained at or above control levels during arrest in animals receiving either unmodified blood or glutamate-enriched crystalloid cardioplegia. While glutamate significantly improved the ability of the crystalloid solution to preserve ATP during arrest, when added to blood, it contributed to a depressed ATP after a three-hour arrest.
Creatine phosphate declined during arrest in all animals, but those receiving unenriched blood cardioplegia consistently had the highest levels (p < 0.05). Addition of glutamate to crystalloid cardioplegia provided a significantly (p < 0.05) higher level of CP at the end of three hours of arrest, which was still lower than that noted with unenriched blood. Comparable to its effect on the ATP level, when glutamate was added to blood cardioplegia, a decrease (p < 0.05) in CP was noted after three hours of arrest. Attempts to enhance high-energy phosphate production by supplementing blood cardioplegia with L-glutamate are ineffective, while increased high-energy phosphate production results when glutamate is added to crystalloid cardioplegia. This implies that L-glutamate functions where anaerobic and not aerobic metabolism is the major component of preservation.
With reperfusion, the only group of animals displaying depressed levels of ATP and CP was that receiving glutamate-enriched blood cardioplegia.
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Footnotes
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Presented at the 54th Annual Scientific Session of the American Heart Association, Nov 16–19, 1981, Dallas, TX.
Supported by Grant 1 R01 HL 22559-02A1 from the National Institutes of Health and Grant 80-895 from the American Heart Association.
Assistance in the preparation of this manuscript by Mrs. Jamie Banks is gratefully acknowledged.
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