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Ann Thorac Surg 2003;76:1777
© 2003 The Society of Thoracic Surgeons

Correspondence

Extracellular matrix in tissue-engineered pulmonary arteries: Reply

Stefan Neuenschwander, PhDa, Simon P. Hoerstrup, MDa

a Clinic for Cardiovascular Surgery, University Hospital Zurich, Raemistrasse 100, CH 8091 Zurich, Switzerland

e-mail: simon_philipp.hoerstrup{at}chi.usz.ch

To the Editor:

We agree with Opitz and associates that transmission electron microscopy has methodological limitations in terms of evaluating the specificity and the maturity of extracellular matrix components. Nevertheless, it provides valuable information on the cellular vitality and activity in engineered tissues. A final answer to the question whether the observed structures are myofilaments or collagen fibrils and whether there is elastin detectable would require more extended and specific methods such as immune electron microscopy. However, this very sophisticated method was beyond the scope of the study [1].

References

  1. Hoerstrup S.P., Kadner A., Breymann C., et al. Living, autologous pulmonary artery conduits tissue engineered from human umbilical cord cells. Ann Thorac Surg 2002;74:46-52.[Abstract/Free Full Text]




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