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Ann Thorac Surg 1995;60:36-37
© 1995 The Society of Thoracic Surgeons
| The first 20% of the full text of this article appears below. |
| Introduction |
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DR ALAN G. CASSON (Toronto, Ont, Canada): I have two questions. First, how did you process the primary tumor? Was this a straightforward surgical implantation, or did you homogenize it and come up with a single-cell suspension? Second, was it one mouse per tumor specimen, or did you actually take the specimen and put it into 6 mice or 8 or 10 mice for the first generation?
MR JOHNSON: Thank you for those questions. First when we received the freshly resected tumor in our laboratory, it was coarsely minced; we coarsely minced all of
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