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Ann Thorac Surg 2009;88:1269-1276. doi:10.1016/j.athoracsur.2009.04.087
© 2009 The Society of Thoracic Surgeons
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Hiroaki Takahashi
Takenori Yokota
Takeyoshi Ota
Kenji Okada
Yoshiki Sawa
Yutaka Okita
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Original Articles: Adult Cardiac

Newly Developed Tissue-Engineered Material for Reconstruction of Vascular Wall Without Cell Seeding

Hiroaki Takahashi, MD, PhDa, Takenori Yokota, MD, PhDb, Eiichiro Uchimura, PhDb, Shigeru Miyagawa, MD, PhDb, Takeyoshi Ota, MD, PhDa, Kei Torikai, MD, PhDb, Atsuhiro Saito, PhDb, Koichiro Hirakawa, MSc, Katsukiyo Kitabayashi, MDb, Kenji Okada, MD, PhDa, Yoshiki Sawa, MD, PhDb,*, Yutaka Okita, MD, PhDa

a Department of Surgery, Division of Cardiovascular Surgery, Kobe University Graduate School of Medicine, Kobe, Japan
b Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan
c Senko Medical Instrument Manufacturing Company Ltd, Tokyo, Japan

Accepted for publication April 16, 2009.

* Address correspondence to Dr Sawa, Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka, 565-0871, Japan (Email: sawa{at}surg1.med.osaka-u.ac.jp).

Background: We have developed a tissue-engineered patch for cardiovascular repair. Tissue-engineered patches facilitated site-specific in situ recellularization and required no pretreatment with cell seeding. This study evaluated the patches implanted into canine pulmonary arteries.

Methods: Tissue-engineered patches are biodegradable sheets woven with double-layer fibers. The fiber is composed of polyglycolic acid and poly-L-lactic acid, and compounding collagen microsponges. The patches (20- x 25-mm) were implanted into the canine pulmonary arterial trunks. At 1, 2, and 6 months after implantation (n = 4), they were explanted and characterized by histologic and biochemical analyses. Commercially available patches served as the control. No anticoagulant therapy was administered postoperatively.

Results: No aneurysm or thrombus was present within the patch area in all groups. The remodeled tissue predominantly consisted of elastic and collagen fibers, and the endoluminal surface was covered with a monolayer of endothelial cells and multilayers of smooth muscle cells beneath the endothelial layer. The elastic and collagen fibers and smooth muscle cells kept increasing with a maximum at 6 months, while a monolayer of endothelial cells was preserved. The expression levels of messenger RNA of several growth factors in the tissue-engineered patches were higher than those of native tissue at 1 and 2 months and decreased to normal level at 6 months. No regenerated tissue was found on the endoluminal surface in the control group.

Conclusions: The novel tissue-engineered patches showed in situ repopulation of host cells without prior ex vivo cell seeding. This is promising material for repair of the cardiovascular system.






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