HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Eugene K.W. Sim Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Guo, C. Articles by Sim, E. K.W. Search for Related Content PubMed PubMed Citation Articles by Guo, C. Articles by Sim, E. K.W. Related Collections Molecular biology Transplantation - heart Related Article

---

Original Articles: Adult Cardiac

Myoblast Transplantation for Cardiac Repair: From Automyoblast to Allomyoblast Transplantation

Changfa Guo, MD^a,_*, Husnain Kh. Haider, PhD^b, Chunsheng Wang, MD^a, Ru-San Tan, MBBS^c, Winston S.N. Shim, PhD^c,d, Philip Wong, MBBS^c, Eugene K.W. Sim, FRCS^d,e,

^a Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai, China
^b Department of Pathology and Laboratory Medicine, University of Cincinnati, Cincinnati, Ohio
^c National Heart Center, Singapore
^d Department of Surgery, National University of Singapore, Singapore
^e Gleneagles JPMC Cardiac Center, Brunei Darussalam

Accepted for publication August 14, 2008.

^_* Address correspondence to Dr Guo, Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, 180 Fenglin Rd, Shanghai City, 200032, China (Email: drguocf{at}yahoo.com; sursimkw{at}nus.edu.sg).
A/P Eugene K. W. Sim, Department of Surgery, National University of Singapore, Singapore 117597 (Email: drguocf{at}yahoo.com; sursimkw{at}nus.edu.sg).

Background: We sought to compare host immune cell kinetics, survival profile of donor skeletal myoblasts, and skeletal myoblast graft efficacy after autologous and allogeneic skeletal myoblast transplantation into a rat model of myocardial infarction.

Methods: One week after myocardial infarction, 128 animals were divided into four groups: group 1 (n = 24, receiving medium only), group 2 (n = 24, receiving medium and cyclosporine), group 3 (n = 40, autologous skeletal myoblast transplantation), and group 4 (n = 40, allogeneic skeletal myoblast transplantation with cyclosporine treatment). Rats were euthanized 10 minutes, 1 day, and 4, 7, and 28 days later. Host immune cell kinetics were assessed by immunohistochemical studies for macrophages, and CD4+ and CD8+ lymphocytes. Donor skeletal myoblast survival was confirmed by tracking prelabeled signals, and quantified by β-gal assay. Heart function was evaluated by echocardiography.

Results: A transient immune cell infiltration was demonstrated in group 3, with macrophage infiltration on day 1 and day 4, CD8+ cell infiltration on day 4 and day 7, and CD4+ cell infiltration on day 4. In group 4, immunocyte infiltration was slightly more severe than that in group 3. Automyoblasts and allomyoblasts showed no significant difference of survival from day 1 to day 7 (p > 0.10); however, on day 28, automyoblasts showed better survival than allomyoblasts (p < 0.05). Transplantation of allomyoblasts increased systolic heart function and limited heart dilation after myocardial injury to a similar degree as automyoblasts (p > 0.10).

Conclusions: The use of allomyoblasts is feasible and effective for cardiac repair with immunosuppressive treatment as compared with automyoblasts.

Related Article

Invited Commentary

Ray C.J. Chiu
Ann. Thorac. Surg. 2008 86: 1848. [Extract] [Full Text] [PDF]

This article has been cited by other articles:

				R. C.J. Chiu Invited Commentary Ann. Thorac. Surg., December 1, 2008; 86(6): 1848 - 1848. [Full Text] [PDF]