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Original Articles: Cardiovascular

Heat Shock Proteins 27, 60, 70, 90, and 20S Proteasome in On-Pump Versus Off-Pump Coronary Artery Bypass Graft Patients

^a Department of Cardiac Surgery, University of Debrecen, Debrecen, Hungary
^b Department of Cardiothoracic Surgery, Medical University of Vienna, Vienna, Austria
^c Department of Cardiothoracic Anaesthesia, Medical University of Vienna, Vienna, Austria

Accepted for publication June 15, 2007.

^_* Address correspondence to Dr Ankersmit, Department of Cardiothoracic Surgery, Medical University of Vienna, Währinger Gürtel 18–20, Vienna, 1090, Austria (Email: hendrik.ankersmit{at}meduniwien.ac.at).

Background: The secretion of heat shock protein (HSP) 27, HSP60, HSP70, HSP90, 20S proteasome, and their correlations to proinflammatory cytokine interleukin-6 is unknown in patients undergoing on-pump versus off-pump coronary artery bypass graft (CABG) operation.

Methods: Forty patients were included in this explorative study (on- versus off-pump CABG, each n = 20). Serum samples were obtained before and 30 minutes, 60 minutes, and 24 hours after CABG operation. Enzyme-linked immunosorbent assay technique was utilized to determine soluble HSP27, 60, 70, and 90, 20S proteasome, and levels of interleukin-6.

Results: Serum levels of HSP are increased in patients undergoing on-pump CABG operation as compared with off-pump CABG technique. These differences were highly significant for HSP27, 70, and 90 at 60 minutes after initiation of cardiopulmonary bypass (all, p < 0.001). Concentrations of soluble 20S proteasome were increased 24 hours after operation in on- and off-pump CABG patients (p < 0.001) and correlated significantly with the serum content of HSP 27, 70, and 90 at 60 minutes after initiation of cardiopulmonary bypass (p < 0.001). No correlation was found when comparing interleukin-6 levels with intravascular leakage of HSP and 20S proteasome after CABG operation.

Conclusions: We conclude from our data that the innate immune system is activated owing to spillage of known immune modulatory and apoptosis-associated proteins after CABG operation.

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