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Ann Thorac Surg 2007;83:1484-1490
© 2007 The Society of Thoracic Surgeons

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Original Articles: Cardiovascular

Therapeutic Benefit of Intrathecal Injection of Marrow Stromal Cells on Ischemia-Injured Spinal Cord

^a Department of Cardiac Surgery, First Affiliated Hospital, China Medical University, Shenyang, China
^b First Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan
^c Department of Anesthesiology, Hamamatsu University School of Medicine, Hamamatsu, Japan

Accepted for publication November 16, 2006.

^_* Address correspondence to Dr Shi, Department of Cardiac Surgery, First Affiliated Hospital, China Medical University, 155 Nanjingbei St, Shenyang 110001, China (Email: shienyi2002{at}hotmail.com).

Presented at the Poster Session of the Forty-third Annual Meeting of The Society of Thoracic Surgeons, San Diego, CA, Jan 29–31, 2007.

Background: Prophylactic transplantation of marrow stromal cells (MSCs) before spinal cord ischemia has been shown to attenuate neurologic injures. We sought to investigate the therapeutic effect of MSCs on ischemia-injured spinal cord.

Methods: Marrow stromal cells were expanded in vitro and prelabeled with bromodeoxyuridine. Spinal cord ischemia was induced in rabbits by infrarenal aortic occlusion for 30 minutes. Four groups were enrolled. About 1 x 10⁸ MSCs were intrathecally injected 2 hours (group MSC-2h), 24 hours (group MSC-24h), or 48 hours (group MSC-48h) after spinal cord ischemia, respectively. The control group received intrathecal injection of medium alone. Hind-limb motor function was assessed during a 28-day recovery period with Tarlov criteria, and then histologic examination was performed.

Results: Marrow stromal cells still could be found in the spinal cord 4 weeks after transplantation. The capillary density in the ventral gray matter was significantly increased in the three MSC-treated groups (p < 0.01 versus control group, respectively). After a 28-day recovery, marked functional improvement was detected in group MSC-2h (from day 1 to 28, p < 0.05, versus control group, respectively) and group MSC-24h (from day 14 to 28, p < 0.05, versus control group, respectively), but not in group MSC-48h. The number of intact motor neurons was much greater in group MSC-2h (p < 0.05, versus control group).

Conclusions: Intrathecal injection of MSCs enhances angiogenesis in the host spinal cord and improves the motor functional recovery after spinal cord ischemia. The therapeutic time window is critical for the therapeutic effect of MSCs.

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Invited commentary

Tatu Juvonen and Petri Lehenkari
Ann. Thorac. Surg. 2007 83: 1490. [Extract] [Full Text] [PDF]

This article has been cited by other articles:

				T. Juvonen and P. Lehenkari Invited commentary Ann. Thorac. Surg., April 1, 2007; 83(4): 1490 - 1490. [Full Text] [PDF]