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Ann Thorac Surg 2006;82:1085-1088
© 2006 The Society of Thoracic Surgeons

New technology

A Novel Method for Preserving Human Lungs Using a Super-Cooling System

Masayoshi Abe, MD, PhDa,*, Shiro Jimi, PhDd, Hiroshi Hamac, Takeshi Shiraishi, MD, PhDb, Akinori Iwasaki, MD, PhDb, Nobuhumi Ono, PhDc, Takayuki Shirakusa, MD, PhDb, Takeshi Katsuragi, PhDa

a Department of Pharmacology, Fukuoka University, Fukuoka, Japan
b Second Department of Surgery, Fukuoka University, Fukuoka, Japan
c Laboratory for Pathology and Morphology, School of Medicine, Fukuoka University, Fukuoka, Japan
d Department of Drug Information, School of Pharmaceutical Science, Fukuoka University, Fukuoka, Japan

Accepted for publication March 3, 2006.

* Address correspondence to Dr Abe, Department of Pharmacology, School of Medicine, Fukuoka University, Fukuoka, 814-0180 Japan (Email: abemasa{at}fukuoka-u.ac.jp).

PURPOSE: To ensure the suitable preservation of isolated lungs, a super-cooling system was used to cool water to temperatures as low as –5°C without freezing.

DESCRIPTION: After lung tissues were obtained from patients with lung cancer, they were kept at –5°C or 4°C for as many as 5 days, and then they were histologically and biochemically examined. To evaluate biochemical stability, tissues after storage were passively sensitized with immunoglobulin E and then incubated with anti-immunoglobulin-E antibody.

EVALUATION: Although tissues preserved at –5°C for 5 days had an almost normal appearance with intact cilia on bronchial epithelium and normal endothelium, tissues stored at 4°C showed degradation of these structures. Single-stranded DNA, a sign of DNA cleavage, was frequently noted in tissues stored at 4°C, but only rarely observed at –5°C. A significant amount of cysteinyl-leukotrienes was generated from tissues stored at –5°C for 3 days, but there was no response to antibody stimulation from tissues stored at 4°C.

CONCLUSIONS: Super-cooling systems may provide useful applications as a novel preserving method.


Related Article

Invited commentary
Charles Hoopes
Ann. Thorac. Surg. 2006 82: 1088-1089. [Extract] [Full Text] [PDF]



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C. Hoopes
Invited commentary.
Ann. Thorac. Surg., September 1, 2006; 82(3): 1088 - 1089.
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