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Ann Thorac Surg 2006;81:2227-2234
© 2006 The Society of Thoracic Surgeons

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Original article: Cardiovascular

Intrathecal Injection of Bone Marrow Stromal Cells Attenuates Neurologic Injury After Spinal Cord Ischemia

^a First Department of Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan
^b Department of Anesthesiology, Hamamatsu University School of Medicine, Hamamatsu, Japan

Accepted for publication December 16, 2005.

^_* Address correspondence to Dr Kazui, First Department of Surgery, Hamamatsu University School of Medicine, 1-20-1 Handayama, Hamamatsu, 431-3192 Japan (Email: tkazui{at}hama-med.ac.jp).

Presented at the Forty-second Annual Meeting of The Society of Thoracic Surgeons, Chicago, IL, Jan 30–Feb 1, 2006.

BACKGROUND: It has been shown that transplantation of bone marrow stromal cells (MSCs) into the ischemic brain improves functional outcome. We sought to investigate whether intrathecal injection of MSCs can attenuate neurologic injury of spinal cord ischemia.

METHODS: Rabbit MSCs were expanded in vitro and were pre-labeled with bromodeoxyuridine. Spinal cord ischemia was induced in rabbits by infrarenal aortic occlusion. Group A and control A were subjected to a 20-minute ischemia and the ischemic duration was extended to 30 minutes in group B and control B. Two days before spinal cord ischemia, 1x10⁸ MSCs were intrathecally injected into groups A and B, whereas vehicle alone was injected into the control groups. Hind-limb motor function was assessed during a 14-day recovery period with Tarlov criteria, and then histologic examination was performed.

RESULTS: Marrow stromal cells survived and engrafted into the spinal cord 2 days after transplantation, and more MSCs were found in the lumbar spinal cord (ischemic segment) than in the thoracic spinal cord (nonischemic segment) at 14 days. Compared with their respective control groups, Tarlov scores were significantly higher in both groups A and B (p < 0.05, group A vs control A, at 2, 7, and 14 days; p < 0.05, group B vs control B, at 1, 2, 7, and 14 days, respectively). The number of intact motor neurons was much higher in the two experimental groups (p < 0.01 vs the corresponding control groups, respectively).

CONCLUSIONS: Intrathecal injection of MSCs attenuates ischemic injury of spinal cord.

This article has been cited by other articles:

				E. Shi, T. Kazui, X. Jiang, N. Washiyama, K. Yamashita, H. Terada, and A. H. M. Bashar Therapeutic Benefit of Intrathecal Injection of Marrow Stromal Cells on Ischemia-Injured Spinal Cord Ann. Thorac. Surg., April 1, 2007; 83(4): 1484 - 1490. [Abstract] [Full Text] [PDF]

				E. Shi, X. Jiang, T. Kazui, N. Washiyama, K. Yamashita, H. Terada, and A. H. M. Bashar Nonviral gene transfer of hepatocyte growth factor attenuates neurologic injury after spinal cord ischemia in rabbits J. Thorac. Cardiovasc. Surg., October 1, 2006; 132(4): 941 - 947. [Abstract] [Full Text] [PDF]