| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Ann Thorac Surg 2005;79:1522-1528
© 2005 The Society of Thoracic Surgeons
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Research Center for Traumatology AUVA, Vienna, Austria
Accepted for publication November 2, 2004.
* Address reprint requests to Mr Fürst, Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, Donaueschingenstrasse 13, A-1200 Vienna, Austria (E-mail: office{at}lbitrauma.org).
BACKGROUND: A two-component sealant composed of bovine serum albumin and glutaraldehyde (BioGlue) is used to treat aortic dissections. Although glutaraldehyde guarantees strong adherence to tissues and synthetic materials, its toxic potential should be considered. The aim of this study was to determine the amount of glutaraldehyde released from BioGlue, its cytotoxic effects on cultured cells, and the local reaction of lung, liver, and aortic tissues to BioGlue.
METHODS: BioGlue was prepared according to the product insert, allowed to polymerize, and then overlaid with saline solution. The supernatant was analyzed for its content of glutaraldehyde. The cytotoxic effect of BioGlue was evaluated by adding the supernatants to either cultured human embryo fibroblasts (MRC5) or mouse myoblasts (C2C12). In vivo toxicity was assessed on three different tissues by applying BioGlue onto a partial lung resection, a liver abrasion, or an intact abdominal aorta in rabbits. Tissue samples were histologically evaluated 2 and 7 days after application.
RESULTS: Saline supernatants from polymerized BioGlue contained 100 to 200 µg/mL glutaraldehyde and were cytotoxic to both cell lines tested. Application of BioGlue to lung and liver tissue evoked serious adverse effects consisting of high-grade inflammation, edema, and toxic necrosis. Intact aortic tissue showed only low-grade or medium-grade inflammation.
CONCLUSIONS: Polymerized BioGlue releases amounts of glutaraldehyde that are capable of inducing cytotoxic effects both in vitro and in vivo. Use of BioGlue should be restricted to the aortic dissection procedure, as other tissues are sensitive to the amounts of glutaraldehyde released from the glue.
This article has been cited by other articles:
|
|
 |
|
  A. N. Azadani, P. B. Matthews, L. Ge, Y. Shen, C.-S. Jhun, T. S. Guy, and E. E. Tseng Mechanical Properties of Surgical Glues Used in Aortic Root Replacement Ann. Thorac. Surg., April 1, 2009; 87(4): 1154 - 1160. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Komiya, N. Tamura, G. Sakaguchi, and T. Kobayashi Modified partial aortic root remodeling in acute type A aortic dissection Interactive CardioVascular and Thoracic Surgery, March 1, 2009; 8(3): 306 - 309. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. J. Zehr Reply. Ann. Thorac. Surg., September 1, 2008; 86(3): 1056 - 1057. [Full Text] [PDF]
|
|
|
|
 |
|
 M. N. Helmus, D. F. Gibbons, and D. Cebon Biocompatibility: Meeting a Key Functional Requirement of Next-Generation Medical Devices Toxicol Pathol, January 1, 2008; 36(1): 70 - 80. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. B. Reece, G. R. Green, and I. L. Kron Aortic Dissection Card. Surg. Adult, January 1, 2008; 3(2008): 1195 - 1222. [Full Text]
|
|
|
|
|
|
D. Spielvogel, M. N. Mathur, and R. B. Griepp Aneurysms of the Aortic Arch Card. Surg. Adult, January 1, 2008; 3(2008): 1251 - 1276. [Full Text]
|
|
|
|
 |
|
 W. Schiller, H. Rudorf, C. B. Welzel, M. J. Kiderlen, C. Probst, O. Dewald, and A. Welz Sutureless anastomoses of rabbit carotid arteries with BioGlue. J. Thorac. Cardiovasc. Surg., December 1, 2007; 134(6): 1513 - 1518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. J. Van Nooten, P. Somers, R. Forsyth, K. Narine, Y. Van Belleghem, S. Jacobs, and F. De Somer Autologous glue: Part of the sticky mystery unraveled J. Thorac. Cardiovasc. Surg., August 1, 2007; 134(2): 415 - 423. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakajima, K. Kawazoe, T. Kataoka, H. Kin, T. Kazui, H. Okabayashi, and H. Niinuma Midterm Results of Aortic Repair Using a Fabric Neomedia and Fibrin Glue for Type A Acute Aortic Dissection Ann. Thorac. Surg., May 1, 2007; 83(5): 1615 - 1620. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Hata, H. Takano, G. Matsumiya, N. Fukushima, N. Kawaguchi, and Y. Sawa Late Complications of Gelatin-Resorcin-Formalin Glue in the Repair of Acute Type A Aortic Dissection Ann. Thorac. Surg., May 1, 2007; 83(5): 1621 - 1626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Haj-Yahia, T. Mittal, E. Birks, M. Carby, M. Petrou, J. Pepper, G. Dreyfus, and M. Amrani Lung fibrosis as a potential complication of the hemostatic tissue sealant, biologic glue (Bioglue) J. Thorac. Cardiovasc. Surg., May 1, 2007; 133(5): 1387 - 1388. [Full Text] [PDF]
|
|
|
|
|
|
P. Tansley, F. Al-Mulhim, E. Lim, G. Ladas, and P. Goldstraw A prospective, randomized, controlled trial of the effectiveness of BioGlue in treating alveolar air leaks J. Thorac. Cardiovasc. Surg., July 1, 2006; 132(1): 105 - 112. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Szafranek, S.R. Podila, W. Al-Khyatt, and E.N. Kulatilake Aseptic mediastinal cyst caused by BioGlue 7 months after cardiac surgery J. Thorac. Cardiovasc. Surg., May 1, 2006; 131(5): 1202 - 1203. [Full Text] [PDF]
|
|
|
|
|
|
W. Furst and H. Redl Conflict of Interest Disclosure Relating to "Release of Glutaraldehyde From an Albumin-Glutaraldehyde Tissue Adhesive Causes Significant In Vitro and In Vivo Toxicity. Ann Thorac Surg 2005;79:1522-8." Ann. Thorac. Surg., September 1, 2005; 80(3): 1159 - 1159. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ANN THORAC SURG | ASIAN CARDIOVASC THORAC ANN | EUR J CARDIOTHORAC SURG |
| J THORAC CARDIOVASC SURG | ICVTS | ALL CTSNet JOURNALS |
