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Justus T. Strauch
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Raimund Bruhin
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Ann Thorac Surg 2005;79:e21-e22
© 2005 The Society of Thoracic Surgeons

Case report

Polymerase Chain Reaction Amplification as a Diagnostic Tool in Culture-Negative Multiple-Valve Endocarditis

Navid Madershahian, MDa, Justus T. Strauch, MDa,*, Martin Breuer, MDa, Raimund Bruhin, MDa, Eberhard Straube, MDb, Thorsten Wahlers, MDa

a Department of Cardiothoracic and Vascular Surgery, Friedrich-Schiller University Jena, Jena, Germany
b Institute of Medical Microbiology, Friedrich-Schiller University Jena, Jena, Germany

Accepted for publication February 10, 2004.

* Address reprint requests to Dr Madershahian, Department of Cardiothoracic and Vascular Surgery, Friedrich-Schiller University Jena, Bachstrasse 18, 07743 Jena, Germany
navid.madershahian{at}med.uni-jena.de

We report a case of culture-negative infectious endocarditis in a 17-year-old boy in which the etiologic diagnosis could only be provided by polymerase chain reaction amplification and sequencing of the bacterial 16S rRNA gene from valve tissue.






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