ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Ara Ketchedjian
Richard Hopkins
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ketchedjian, A.
Right arrow Articles by Hopkins, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ketchedjian, A.
Right arrow Articles by Hopkins, R.
Related Collections
Right arrow Molecular biology
Right arrowRelated Article

Ann Thorac Surg 2005;79:888-896
© 2005 The Society of Thoracic Surgeons

Original article: Cardiovascular

Recellularization of Decellularized Allograft Scaffolds in Ovine Great Vessel Reconstructions

Ara Ketchedjian, MDa, Alyce Linthurst Jones, MSb, Paula Krueger, BSa, Elliot Robinson, BSa, Katrina Crouch, BSb, Lloyd Wolfinbarger, Jr, PhDb, Richard Hopkins, MDa,*

a Department of Cardiothoracic Surgery and the Collis Cardiac Surgical Laboratory, Brown University and Rhode Island Hospital, Providence, Rhode Island
b LifeNet Tissue Services, Virginia Beach, Virginia

Accepted for publication September 2, 2004.

* Address reprint requests to Dr Hopkins, Rhode Island and Hasbro Children's Hospital, 2 Dudley Street, MOC #500, Providence, RI 02905; (E-mail: rahopkins{at}lifespan.org).

BACKGROUND: Decellularized allograft tissues have been identified as a potential extracellular matrix (ECM) scaffold on which to base recellularized tissue-engineered vascular and valvular substitutes. Decreased antigenicity and the capacity to recellularize suggest that such constructs may have favorable durability. Detergent/enzyme decellularization methods remove cells and cellular debris while leaving intact structural protein "scaffolds." Allograft pulmonary artery tissues decellularized with an anionic detergent/enzyme methodology were tested in a long-term implantation model that used arterial wall repairs in the great vessels of juvenile sheep.

METHODS: Twelve test sheep were implanted (n = 4) for each of three different scaffold protocols that compared traditional dimethylsulfoxide cryopreservation, cryopreservation followed by decellularization, and decellularization of fresh tissue. Four additional sheep served as controls (n = 2 sham, n = 2 fresh tissue). Patches were fashioned and implanted into pulmonary artery and aortic defects. Panel reactive antibodies (PRA) were measured over time (10 to 20 weeks). Explant histopathology determined recellularization morphology as well as calcium, collagen, and elastin distribution within explanted tissue.

RESULTS: Unlike traditionally cryopreserved tissues, the decellularized tissues contained no residual cells or cellular debris before implantation, which correlated with measurable reductions in PRA. Decellularized explants demonstrated time-dependent migration of recipient cells through matrix, typically staining positive for {alpha}-smooth muscle actin with no calcification.

CONCLUSIONS: The properties demonstrated seem consistent with characteristics necessary for implantable tissue-engineered scaffolds. The decellularization method described appears to create a biologically suitable ECM scaffold for in vivo migration of phenotypically appropriate cells while avoiding antigenicity and calcification.


Related Article

INVITED COMMENTARY
Paul Simon, Marie-Theres Kasimir, Erwin Rieder, and Guenter Weigel
Ann. Thorac. Surg. 2005 79: 896. [Extract] [Full Text] [PDF]



This article has been cited by other articles:


Home page
 Eur Heart JHome page
G. De Visscher, I. Vranken, A. Lebacq, C. Van Kerrebroeck, J. Ganame, E. Verbeken, and W. Flameng
In vivo cellularization of a cross-linked matrix by intraperitoneal implantation: a new tool in heart valve tissue engineering
Eur. Heart J., June 1, 2007; 28(11): 1389 - 1396.
[Abstract] [Full Text] [PDF]

Home page
 JBJSHome page
K. A. Derwin, A. R. Baker, R. K. Spragg, D. R. Leigh, and J. P. Iannotti
Commercial Extracellular Matrix Scaffolds for Rotator Cuff Tendon Repair. Biomechanical, Biochemical, and Cellular Properties
J. Bone Joint Surg. Am., December 1, 2006; 88(12): 2665 - 2672.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
R. A. Hopkins
Bioprosthetic Valves and Laudable Inflammation?
Circulation, July 25, 2006; 114(4): 261 - 264.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2005 by The Society of Thoracic Surgeons.