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Ann Thorac Surg 2004;78:1409-1417
© 2004 The Society of Thoracic Surgeons

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Original article: cardiovascular

Repair of Myocardial Infarction by Epicardial Deposition of Bone Marrow Cell-Coated Muscle Patch in a Murine Model

^a Department of Cardiovascular Surgery and Cardiology, Haut-Lévêque Hospital, Pessac, France
^b National Health Institute and Medical Research, Pessac, and University of Bordeaux 2 - Victor Segalen, Bordeaux, Cedex, France

Accepted for publication December 29, 2003.

^* Address reprint requests to Dr Barandon, Cardiovascular Surgery Department, Haut-Lévêque Hospital, 33604 Pessac, France
lesbarandon{at}wanadoo.fr

BACKGROUND: Myocardial infarction results in irreversible myocyte loss. In a murine model, we tested the feasibility of a novel repair technique combining bone marrow cell (BMC) transplantation and cardiomyoplasty.

METHODS: Myocardial infarction was induced cryogenically in backcrossed ROSA 26 transgenic x C57BL/6J mice (n = 75). Thirty days later, surviving mice (n = 69) were randomized to sham treatment (rethoracotomy only; n = 11), patch only treatment (n = 29), or patch + BMC treatment (n = 29). Abdominal muscle patches were harvested from donor littermates not expressing the ß-galactosidase reporter gene and sutured on the epicardium directly above the infarct zone. Patch only–treated mice received uncoated patches. Patch + BMC–treated mice received patches coated with 5 x 10⁶ ß-galactosidase-expressing BMCs embedded in a collagen-rich three-dimensional matrix.

RESULTS: Mortality rate was 52% after muscle patch implantation. Bone marrow cells were able to migrate from muscle patch into the infarct zone, as demonstrated by ß-galactosidase immunostaining, and ultimately constituted 8% of all cells in scar tissue (mean ± standard deviation, 219 ± 111/mm²). Angiogenesis and cell survival in the scar were improved by patch + BMC treatment. Left ventricular geometry and cardiac function were improved by patch treatment, with or without BMC, although the effects were stronger after patch + BMC treatment.

CONCLUSIONS: Epicardial deposition of a BMC-coated muscle patch is a promising approach to restoring cardiac function after myocardial infarction.

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