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Ann Thorac Surg 2004;78:970-975
© 2004 The Society of Thoracic Surgeons

Original article: cardiovascular

Pinacidil improves contractile function and intracellular calcium handling in isolated cardiac myocytes exposed to simulated cardioplegic arrest

Ru Lin, MD, PhDa,*, Ze-Wei Zhang, MDa, Qi-Xing Xiong, MDa, Chun-Mei Cao, PhDb, Qiang Shu, MDa, Iain C. Bruce, PhDc, Qiang Xia, PhDb

a Department of Cardiothoracic Surgery, Children's Hospital, Zhejiang University, Hangzhou, China
b Department of Physiology, Zhejiang University School of Medicine, Hangzhou, China
c Department of Physiology, The University of Hong Kong, Hong Kong, China

Accepted for publication March 30, 2004.

* Address reprint requests to Dr Lin, Department of Cardiothoracic Surgery, Children's Hospital, Zhejiang University, 57 Zhu Gan Xiang, Hangzhou 310003, China
linru.008{at}163.com

BACKGROUND: We examined the effects of pinacidil on contractile function and intracellular calcium in isolated rat cardiomyocytes exposed to cardioplegic solution.

METHODS: Rat myocytes were incubated at 24°C for 2 hours in cardioplegic solution with or without pinacidil (50 µmol/L), then they were perfused with Krebs-Henseleit solution with a gas phase of 95% O2/5% CO2 at the same temperature. Contraction and intracellular calcium transients were then measured by video tracking and spectrofluorometry.

RESULTS: During 20 minutes of perfusion after 2 hours in cardioplegic solution with pinacidil, (1) the recovery of contractile function was significantly increased in terms of both amplitude of contraction (98.30% ± 9.90% versus 81.00% ± 11.25%; p < 0.05) and peak velocity of cell shortening (100.90% ± 13.79% versus 76.89% ± 18.14%; p < 0.01) when compared with myocytes in cardioplegic solution without pinacidil; (2) the amplitudes of the intracellular calcium transients evoked by electrical stimulation and caffeine (10 mmol/L) increased by 23.31% to approximately 40.72% and 61.73%, respectively, compared with those in cardioplegic solution without pinacidil; and (3) the decay time of the caffeine-induced intracellular calcium transient decreased by 36.64% ± 15.10% relative to that measured in cardioplegic solution without pinacidil. The effects induced by supplementing the cardioplegic solution with pinacidil were diminished in the presence of glibenclamide (10 µmol/L).

CONCLUSIONS: Addition of the adenosine triphosphate–sensitive potassium-channel opener, pinacidil, to a high potassium cardioplegic solution improves recovery of contractile properties and cytosolic calcium in isolated rat cardiac myocytes.






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