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Ann Thorac Surg 2003;76:1623-1630
© 2003 The Society of Thoracic Surgeons
a Department of Surgery, Division of Cardiothoracic Surgery, Chinese University of Hong Kong, Hong Kong SAR, China
b Providence Heart Institute, Albert Starr Academic Center, Department of Surgery, Oregon Health and Science University, Portland, Oregon, USA
Accepted for publication April 23, 2003.
* Address reprint requests to Prof He, Department of Surgery, Block B, 5A, Prince of Wales Hospital, Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China
e-mail: gwhe{at}cuhk.edu.hk
BACKGROUND: We examined the effect of 11,12-epoxyeicosatrienoic acid (EET11,12) added to St. Thomas’ Hospital (ST) solution or University of Wisconsin (UW) solution on endothelium-derived hyperpolarizing factor (EDHF)–mediated relaxation under clinically relevant temperature and exposure time.
METHODS: Porcine coronary microarteries (200 to 450 µm) were incubated with Krebs’ solution (control), ST with or without EET11,12 (300 nmol/L) at 22°C for 1 hour as well as at 4°C for 1 or 4 hours, and UW with or without EET11,12 at 4°C for 4 hours. The EDHF-mediated relaxation was induced by bradykinin (-10 to approximately -6.5 log M) in the precontraction evoked by U46619 (10 nmol/L) or U46619 (1 nmol/L) plus endothelin-1 (6 nmol/L).
RESULTS: The EDHF-mediated relaxation was reduced after exposure to UW (79.7% ± 4.6% versus 93.6% ± 2.8%, p = 0.01) at 4°C for 4 hours. One-hour exposure to ST under 22°C or 4°C decreased the relaxation (75.2% ± 7.6% versus 96.7% ± 1.6%, p < 0.05) or the sensitivity to bradykinin (-8.04 ± 0.15 versus -8.50 ± 0.20 log M, p < 0.05). The relaxation increased to 86.8% ± 5.3% by addition of EET11,12 to ST (1 hour at 22°C, p < 0.05) but was unchanged when added to either ST or UW at 4°C for 1 or 4 hours.
CONCLUSIONS: As an additive to ST solution, EET11,12 may partially restore EDHF-mediated endothelial function under moderate hypothermia but had no significant effect under profound hypothermia when added to either ST or UW solution. Further investigation is necessary to improve the effect.
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