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Ann Thorac Surg 2002;73:1606-1614
© 2002 The Society of Thoracic Surgeons

Original article: general thoracic

Endothelial cell injury induced by preservation solutions: a confocal microscopy study

Francesco Alamanni, MDa,*, Alessandro Parolari, MD, PhD*a,*, Rossana Visigalli, BS, PhDb, Ovidio Bussolati, MD, PhDb, Patrizia Rubini, MD, PhDc, Roberto Sala, MD, PhDb, Luigi Bonati, MDc, Gian Carlo Gazzola, MDb, Paolo Biglioli, MDa, Valeria Dall’Asta, BSb

a Department of Cardiac Surgery, Centro Cardiologico-Fondazione I Monzino IRCCS, University of Milan, Milan, Italy
b Department of Experimental Medicine, Unit of General and Clinical Pathology, University of Milan, Milan, Italy
c Institute of General Surgery and Organ Transplantation, University of Parma, Parma, Italy

Accepted for publication January 21, 2002.

* Address reprint requests to Dr Parolari, Department of Cardiac Surgery, University of Milan, Centro Cardiologico-Fondazione I Monzino IRCCS, Via Parea 4, 20138 Milan, Italy
e-mail: alessandro.parolari{at}cardiologicomonzino.it

Background. We evaluated the effects of standard preservation solutions on cultured human greater saphenous vein endothelial cells.

Methods. Endothelial cells (eight strains) were preincubated for 6 or 24 hours at 4°C in Celsior, Euro-Collins, St. Thomas Hospital II, and University of Wisconsin solutions, reincubated in warm oxygenated culture medium 199, and observed up to 48 hours. Culture viability was assessed through cell counting and confocal microscopy of calcein loaded cells.

Results. Incubation in both Euro-Collins and St. Thomas, but not in Celsior or University of Wisconsin solutions, caused significant cells losses and diffuse morphological damages characterized by solution-specific distinctive alterations. Injury caused by 6-hour, but not by 24-hour treatment, was reversible.

Conclusions. The incubation with Celsior and University of Wisconsin solutions substantially preserved endothelial viability and proliferative capability. Conversely, a prolonged incubation in either Euro-Collins or St. Thomas solutions caused severe and potentially irreversible damage referable to the induction of, respectively, apoptotic or necrotic changes.


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Invited commentary
Paul W.M. Fedak, Richard D. Weisel, Subodh Verma, and Vivek Rao
Ann. Thorac. Surg. 2002 73: 1614-1615. [Extract] [Full Text] [PDF]



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