ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Robert M. Mentzer, Jr
Robert D. Lasley
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stevens, R. M.
Right arrow Articles by Lasley, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stevens, R. M.
Right arrow Articles by Lasley, R. D.
Related Collections
Right arrow Myocardial protection

Ann Thorac Surg 2002;73:1261-1266
© 2002 The Society of Thoracic Surgeons

Original article: cardiovascular

Effects of in vivo myocardial ischemia and reperfusion on interstitial nitric oxide metabolites

Randy M. Stevens, MDa, M. Salik Jahania, MDa, Jennifer E. Stivers, BSa, Robert M. Mentzer, Jr, MDa, Robert D. Lasley, PhD*a

a Department of Surgery, University of Kentucky College of Medicine, Lexington, Kentucky, USA

Accepted for publication December 17, 2001.

* Address reprint requests to Dr Lasley, Department of Surgery, University of Kentucky College of Medicine, MN276, Chandler Medical Center, 800 Rose St, Lexington, KY 40536-0298 USA
e-mail: rlasley{at}pop.uky.edu

Background. There have been numerous studies examining the role of nitric oxide (NO) in myocardial ischemia-reperfusion injury; however, few studies have included measurements of NO or related reactive nitrogen species. The purpose of this study was to determine the effects of in vivo regional myocardial ischemia on interstitial fluid (ISF) reactive nitrogen species.

Methods. Open chest pigs were submitted to one of three protocols: (1) 15 minutes coronary occlusion and 2 hours reperfusion, (2) 60 minutes coronary occlusion and 2 hours reperfusion, or (3) two-cycle ischemic preconditioning (IPC) followed by prolonged ischemia and 2 hours reperfusion. The stable NO metabolites, nitrite plus nitrate (NOx), in cardiac microdialysis samples were measured by ozone chemiluminescence.

Results. NOx concentration decreased 40% ± 6% (p < 0.05) during brief ischemia but returned to baseline during reperfusion. Dialysate NOx levels decreased further after 60 minutes ischemia (60% ± 3% of baseline, p < 0.01) but reperfusion dialysate NOx concentration increased 34% ± 9% above baseline (p < 0.05). Preconditioning did not increase dialysate NOx but did accelerate the ischemia-induced decrease in NOx levels (p < 0.05). Reperfusion NOx levels in preconditioned pigs were significantly lower than in nonpreconditioned pigs (p < 0.05).

Conclusions. These results suggest that ischemia is associated with decreased ISF NOx concentration. Reperfusion NOx levels are increased after prolonged ischemia, an effect that is significantly blunted by ischemic preconditioning.




This article has been cited by other articles:


Home page
 HeartHome page
J Trevelyan, E W A Needham, A Morris, and R K Mattu
Comparison of the effect of enalapril and losartan in conjunction with surgical coronary revascularisation versus revascularisation alone on systemic endothelial function
Heart, August 1, 2005; 91(8): 1053 - 1057.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2002 by The Society of Thoracic Surgeons.