HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Hikaru Matsuda Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hayashi, Y. Articles by Matsuda, H. Search for Related Content PubMed PubMed Citation Articles by Hayashi, Y. Articles by Matsuda, H. Related Collections Extracorporeal circulation

Ann Thorac Surg 2001;72:149-155
© 2001 The Society of Thoracic Surgeons

---

Original article: cardiovascular

Inducible nitric oxide production is an adaptation to cardiopulmonary bypass-induced inflammatory response

Accepted for publication March 14, 2001.

Address reprint requests to Dr Sawa, Department of Surgery, Course of Interventional Medicine (E1), Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita City, Osaka 565-0871, Japan
e-mail: sawa{at}surg1.med.osaka-u.ac.jp

Background. Cardiopulmonary bypass (CPB) increases nitric oxide (NO) production by the activation of NO synthases (NOS). However, the role of NO from inducible NOS (iNOS) in CPB-induced inflammatory response remains unclear. We examined the effect of a selective iNOS inhibitor, aminoguanidine, on CPB-induced inflammatory response in a rat-CPB model.

Methods. Adult Sprague-Dawley rats underwent 60 minutes of CPB (100 mL · kg^-1 · min^-1, 34°C). Group A (n = 10) received 100 mg/kg of aminoguanidine intraperitoneally 30 minutes before the initiation of CPB, and group B (n = 10) served as controls.

Results. There were significant time-dependent changes in plasma interleukin (IL)-6, IL-8, nitrate + nitrite, the percentage ratio of nitrotyrosine to tyrosine (%NO₂-Tyr, an indicator of peroxynitrite formation), and respiratory index (RI). Three hours after CPB termination, IL-6, IL-8, and RI were significantly higher in group A (IL-6, 397.5 ± 80.6 pg/mL; IL-8, 26.99 ± 6.57 ng/mL; RI, 1.87 ± 0.31) than in group B (IL-6, 316.5 ± 73.9 pg/mL, p <0.05; IL-8, 17.21 ± 3.12 ng/mL, p < 0.01; RI, 1.57 ± 0.24, p < 0.05) although nitrate + nitrite (31.8 ± 4.1 µmol/L) and %NO₂-Tyr (1.15% ± 0.20%) were significantly lower in group A than in group B (nitrate + nitrite, 50.2 ± 5.0 µmol/L, p < 0.01; %NO₂-Tyr, 1.46% ± 0.21%, p < 0.01). Western immunoblot analysis from lung tissue of group A identified marked iNOS inhibition without inhibiting endothelial-constitutive NOS, and neutrophil accumulation in the lung specimens was significantly greater in group A (6.5 ± 0.7/alveoli) than in group B (4.4 ± 0.4/alveoli, p < 0.01).

Conclusions. These results suggest that NO production from iNOS may be an adaptive response for attenuating the CPB-induced inflammatory response.

This article has been cited by other articles:

				Z. Shen, Z. Wang, J. Zhang, and H. Jing Hepatic injury in a rat cardiopulmonary bypass model Interactive CardioVascular and Thoracic Surgery, February 1, 2008; 7(1): 18 - 22. [Abstract] [Full Text] [PDF]

				C. D. Mazer, F. Briet, K. R. Blight, D. J. Stewart, M. Robb, Z. Wang, A. M. Harrington, W. Mak, X. Li, and G. M.T. Hare Increased cerebral and renal endothelial nitric oxide synthase gene expression after cardiopulmonary bypass in the rat J. Thorac. Cardiovasc. Surg., January 1, 2007; 133(1): 13 - 20. [Abstract] [Full Text] [PDF]

				K. Takakura, M. Mizogami, and S. Fukuda Protamine sulfate causes endothelium-independent vasorelaxation via inducible nitric oxide synthase pathway: [Le sulfate de protamine cause un vasorelachement independant de l'endothelium par la voie de l'oxyde nitrique synthase inductible] Can J Anesth, February 1, 2006; 53(2): 162 - 167. [Abstract] [Full Text] [PDF]

				T. B. Andrasi, A. Blazovics, G. Szabo, C. F. Vahl, and S. Hagl Poly(ADP-ribose) polymerase inhibitor PJ-34 reduces mesenteric vascular injury induced by experimental cardiopulmonary bypass with cardiac arrest Am J Physiol Heart Circ Physiol, June 1, 2005; 288(6): H2972 - H2978. [Abstract] [Full Text] [PDF]

				P. Gessler, J. Pfenninger, J.-P. Pfammatter, T. Carrel, O. Baenziger, and C. Dahinden Plasma levels of interleukin-8 and expression of interleukin-8 receptors on circulating neutrophils and monocytes after cardiopulmonary bypass in children J. Thorac. Cardiovasc. Surg., September 1, 2003; 126(3): 718 - 725. [Abstract] [Full Text] [PDF]

				M Chello, P Mastroroberto, G Patti, A D'Ambrosio, M C. Morichetti, G Di Sciascio, and E Covino Simvastatin attenuates leucocyte-endothelial interactions after coronary revascularisation with cardiopulmonary bypass Heart, May 1, 2003; 89(5): 538 - 543. [Abstract] [Full Text] [PDF]

				Y. Hayashi, Y. Sawa, N. Fukuyama, H. Nakazawa, and H. Matsuda Preoperative Glutamine Administration Induces Heat-Shock Protein 70 Expression and Attenuates Cardiopulmonary Bypass-Induced Inflammatory Response by Regulating Nitric Oxide Synthase Activity Circulation, November 12, 2002; 106(20): 2601 - 2607. [Abstract] [Full Text] [PDF]