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Ann Thorac Surg 2001;71:S413-S416
© 2001 The Society of Thoracic Surgeons

Basic research

Effect of ethanol and ether in the prevention of calcification of bioprostheses

Ming Shen, MD, PhDa, Ali Kara-Mostefa, MDb, Lin Chen, MDa, Michel Daudon, PhDb, Marc Thevenin, PhDb, Bernard Lacour, PhDb, Alain Carpentier, MD, PhDa

a Laboratoire d’Etude des Greffes et Prothèses Cardiaques, Hôpital Européen Georges Pompidou, Paris, France
b Laboratoire de Biochimie A, Hôpital Necker, Paris, France

Address reprint requests to Dr Shen, Laboratoire d’Etude des Greffes et Prothèses Cardiaques, Hôpital Broussais, 96 rue Didot, 75014 Paris, France
e-mail: ming.shen{at}brs.ap-hop-paris.fr

Presented at the VIII International Symposium on Cardiac Bioprostheses, Cancun, Mexico, Nov 3–5, 2000.

Background. Lipids play a significant role in the process of calcification of bioprostheses. We assessed whether lipid extraction by ethanol, ether, or a surfactant could mitigate calcification of glutaraldehyde-treated bioprostheses.

Methods. On 200 bovine pericardium samples pretreated with 0.6% glutaraldehyde, lipid extraction was carried out by ethanol, ether, or the tween 80 surfactant, and combinations thereof. The treated tissues were implanted subcutaneously in 50 juvenile rats for 4 and 6 months. Lipids were analyzed by Fourier transform infrared spectrophotometer and chromatography before implantation. Calcium content of implanted tissues was assessed by atomic absorption spectrometer.

Results. Ethanol, ether, or surfactant did mitigate calcification. The most efficient pretreatments were the combination of ethanol and surfactant (calcium content: 15.5 ± 6.8 µg/mg dry tissue after 6 months implantation) or the combination of ethanol, ether, and surfactant (13.1 ± 6.2 µg/mg dry tissue) when compared with surfactant alone (42.9 ± 12.7 µg/mg dry tissue).

Conclusions. Ethanol or the combination of ethanol and ether added to the currently used glutaraldehyde-surfactant treatment further mitigates calcification.






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