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Ann Thorac Surg 2001;71:827-831
© 2001 The Society of Thoracic Surgeons

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Original article: cardiovascular

Stimulation of neutrophil activation during coronary artery bypass grafting: comparison of crystalloid and blood cardioplegia

^a Department of Cardiosurgery, J. Strus Hospital Pozna, Pozna, Poland
^b Department of Intensive Care and Cardiology, Karol Marcinkowski University School of Medical Sciences, Pozna, Poland

Accepted for publication May 24, 2000.

Address reprint requests to Dr Siminiak, Department of Intensive Care and Cardiology, University School of Medical Sciences, 49 Przybyszewskiego St, 60-355 Pozna, Poland
e-mail: mbalin{at}polbox.com

Background. During myocardial ischemia, activation of polymorphonuclear neutrophils (PMNs) results in the production of free oxygen radicals, which increase myocardial injury. It has been shown that PMNs also produce nitric oxide. It is not clear whether PMNs become activated as a result of their direct contact with ischemic/reperfused myocardium or if PMN activation and free oxygen radical production are effects of specific stimuli released during coronary artery bypass grafting (CABG). The aim of the current study was to evaluate plasma-mediated neutrophil stimulation and production of superoxide anion (O) and nitric oxide in patients undergoing CABG, and to verify whether crystalloid and blood cardioplegia can modify such stimulation.

Methods. Coronary sinus, peripheral arterial, and venous plasma samples were collected from 50 patients who underwent CABG and were divided into 2 equal groups which received either crystalloid or blood cardioplegia: directly before myocardial ischemia and aortic cross-clamping; at the beginning of reperfusion after aortic clamp release; and 30 minutes after reperfusion. O and nitric oxide production by PMN was evaluated by standard methods.

Results. There was a significant (p < 0.05) increase in O production by PMN incubated with plasma obtained from the coronary sinus immediately after reperfusion in patients receiving crystalloid cardioplegia compared to blood cardioplegia. No difference was observed in plasma stimulation of nitric oxide production by PMN in the 2 groups of patients at different times during the procedure.

Conclusions. Cardioplegia may affect release of neutrophil-oriented stimuli from ischemic myocardium and modify neutrophil activation during coronary artery bypass grafting.

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