ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Norihide Fukushima
Hajime Ichikawa
Hikaru Matsuda
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakaguchi, T.
Right arrow Articles by Matsuda, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakaguchi, T.
Right arrow Articles by Matsuda, H.
Related Collections
Right arrow Molecular biology
Right arrow Myocardial protection
Right arrowRelated Article

Ann Thorac Surg 2001;71:624-629
© 2001 The Society of Thoracic Surgeons

Original article: cardiovascular

A novel strategy of decoy transfection against nuclear factor-{kappa}B in myocardial preservation

Taichi Sakaguchi, MDa, Yoshiki Sawa, MDa, Norihide Fukushima, MDa, Motonobu Nishimura, MDa, Hajime Ichikawa, MDa, Yasufumi Kaneda, MDb, Hikaru Matsuda, MDa

a Department of Surgery, Course of Interventional Medicine, Osaka University Graduate School of Medicine, Osaka, Japan
b Division of Gene Therapy Science, Osaka University Graduate School of Medicine, Osaka, Japan

Accepted for publication May 10, 2000.

Address reprint requests to Dr Sawa, Department of Surgery, Course of Interventional Medicine (E1), Osaka University Graduate School of Medicine, Yamada-oka 2-2, Suita, Osaka 565-0871, Japan
e-mail: sawa{at}surg1.med.osaka-u.ac.jp

Background. Nuclear factor-{kappa}B (NF{kappa}B) is critical for the transcription of multiple genes involved in myocardial ischemia-reperfusion injury. Therefore, we hypothesized that blocking NF{kappa}B would attenuate ischemia-reperfusion injury after prolonged myocardial preservation, resulting in an improvement in cardiac function.

Methods. Double-stranded oligodeoxynucleotides with a specific affinity for NF{kappa}B (NF{kappa}B decoy group) or a scrambled decoy group were transfected into rat hearts using a hemagglutinating virus of Japan–liposome method. After 16 hours of preservation in Euro-Collins solution at 4°C, the cardiac grafts were heterotopically transplanted into recipient rats of the same strain.

Results. Fluorescein isothiocyanate staining showed introduction of double-stranded oligonucleotides into the nuclei of endothelial cells and cardiomyocytes. After 1 hour of reperfusion the NF{kappa}B decoy group showed significantly higher degrees of recovery of left ventricular function as well as significantly lower levels of serum creatine phosphokinase, myocardial water content, tissue IL-8, and neutrophil infiltration than did the scrambled decoy group (p < 0.05).

Conclusions. Gene transfection of the NF{kappa}B decoy attenuates ischemia-reperfusion injury after prolonged heart preservation. As a result, this method appears to be a novel strategy for enhanced myocardial preservation.


Related Article

Invited commentary
Baiya Krishnadasan and Edward D. Verrier
Ann. Thorac. Surg. 2001 71: 629-630. [Extract] [Full Text] [PDF]



This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
P. L. Hermonat, D. Li, B. Yang, and J. L. Mehta
Mechanism of action and delivery possibilities for TGF{beta}1 in the treatment of myocardial ischemia
Cardiovasc Res, May 1, 2007; 74(2): 235 - 243.
[Abstract] [Full Text] [PDF]

Home page
 Eur. J. Cardiothorac. Surg.Home page
K. Ohmori, S.-i. Takeda, S. Miyoshi, M. Minami, S. Nakane, M. Ohta, Y. Sawa, and H. Matsuda
Attenuation of lung injury in allograft rejection using NF-{kappa}B decoy transfection--novel strategy for use in lung transplantation
Eur. J. Cardiothorac. Surg., January 1, 2005; 27(1): 23 - 27.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Ishii, Y. Suzuki, K. Takeshita, N. Miyao, H. Kudo, R. Hiraoka, K. Nishio, N. Sato, K. Naoki, T. Aoki, et al.
Inhibition of c-Jun NH2-Terminal Kinase Activity Improves Ischemia/Reperfusion Injury in Rat Lungs
J. Immunol., February 15, 2004; 172(4): 2569 - 2577.
[Abstract] [Full Text] [PDF]

Home page
 Eur. J. Cardiothorac. Surg.Home page
D. L. Ngaage
Off-pump coronary artery bypass grafting: the myth, the logic and the science
Eur. J. Cardiothorac. Surg., October 1, 2003; 24(4): 557 - 570.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Interv.Home page
F. D'Acquisto, M. J. May, and S. Ghosh
Inhibition of Nuclear Factor Kappa B (NF-B):: An Emerging Theme in Anti-Inflammatory Therapies
Mol. Interv., February 1, 2002; 2(1): 22 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Eur. J. Cardiothorac. Surg.Home page
D. Paparella, T.M. Yau, and E. Young
Cardiopulmonary bypass induced inflammation: pathophysiology and treatment. An update
Eur. J. Cardiothorac. Surg., February 1, 2002; 21(2): 232 - 244.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2001 by The Society of Thoracic Surgeons.