ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Rephael Mohr
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pevni, D.
Right arrow Articles by Mohr, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pevni, D.
Right arrow Articles by Mohr, R.

Ann Thorac Surg 2000;70:2050-2053
© 2000 The Society of Thoracic Surgeons

Original article: cardiovascular

Protamine induces vasorelaxation of human internal thoracic artery by endothelial NO-synthase pathway

Dmitry Pevni, MDa, Jacob Gurevich, MDa, Inna Frolkis, MD, PhDa, Gad Keren, MDa, Izhak Shapira, MDa, Josef Paz, MDa, Amir Kramer, MDa, Chaim Locker, MDa, Rephael Mohr, MDa

a Department of Thoracic and Cardiovascular Surgery, Tel Aviv Sourasky Medical Center and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel

Accepted for publication March 28, 2000.

Address reprint requests to Dr Mohr, Department of Thoracic and Cardiovascular Surgery, Tel-Aviv Sourasky Medical Center, 6 Weizmann St, Tel-Aviv, Israel 64239
e-mail: shapiraiz{at}tasmc.health.gov.il

Background. Protamine is commonly used in cardiac surgery to reverse the anticoagulant effects of heparin. We investigated the role of different nitric oxide synthase pathways in the response of the human internal thoracic artery to protamine and evaluated whether heparin could prevent this effect.

Methods. A tension-recording method was used to obtain baseline measurements of contractions of human internal thoracic artery rings achieved with norepinephrine. Isolated internal thoracic artery rings were suspended in two organ chambers. One contained Krebs-Henseleit solution and served as control. The other contained a heparin or N{omega}-Nitro-L-arginine (L-NAM, an inhibitor of both endothelial and inducible nitric oxide synthase) or a specific inhibitor of inducible nitric oxide synthase, aminoguanidine. Increasing doses of protamine were added to both chambers and dose-response curves were obtained.

Results. Protamine was found to relax contracted internal thoracic arteries 56% ± 4.7% of baseline measurements in a concentration-dependent manner. When L-NAM was added, protamine caused only a slight decrease of tension. There were no differences in the relaxing effect of protamine in the presence of aminoguanidine or heparin.

Conclusions. Protamine induces nitric oxide-dependent relaxation of the internal thoracic artery by activation of endothelial nitric oxide synthase pathway. Heparin could not prevent this relaxing effect of protamine.




This article has been cited by other articles:


Home page
 Eur. J. Cardiothorac. Surg.Home page
I. Golbasi, C. Nacitarhan, S. Ozdem, C. Turkay, H. Karakaya, G. Sadan, and O. Bayezid
The inhibitory action of protamine on human internal thoracic artery contractions: the effect of free hemoglobin
Eur. J. Cardiothorac. Surg., June 1, 2003; 23(6): 962 - 968.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2000 by The Society of Thoracic Surgeons.