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Ann Thorac Surg 2000;70:1880-1884
© 2000 The Society of Thoracic Surgeons

Original article: general thoracic

Limit of warm ischemia time before cryopreservation in rat tracheal isografts

Ryoichi Nakanishi, MDa, Masayoshi Umesue, MDa, Mitsunori Hashimoto, MDa, Hiroyuki Muranaka, MDa, Mitsuhiro Hachida, MDa, Kosei Yasumoto, MDa

a Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan

Accepted for publication April 6, 2000.

Address reprint requests to Dr Nakanishi, Second Department of Surgery, School of Medicine, University of Occupational and Environmental Health, 1–1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807–8555, Japan
e-mail: ryoichi{at}med.uoeh-u.ac.jp

Background. The viability of cadaveric tracheal grafts undergoing cryopreservation is still unclear. We evaluated the limit of warm ischemia time before cryopreservation in rat tracheal isografts.

Methods. Each isograft was harvested from donor rats 0 to 48 hours (0, 6, 12, 18, 24, and 48 hours) after circulatory arrest, immersed in the preservative solution, and stored in a deep freezer until reaching -80°C and then was kept in liquid nitrogen for 3 months. Heterotopic transplantation into the omentum was performed after the isografts were thawed. Graft morphology 3 months after transplantation was assessed.

Results. The stepwise increase of warm ischemia time significantly reduced graft survival. A prolonged period of warm ischemia had a degenerative effect on both the epithelium and cartilage. The morphology of the epithelium and cartilage in isografts undergoing warm ischemia for less than 18 hours was better preserved, whereas it deteriorated in isografts undergoing warm ischemia for more than 24 hours.

Conclusions. We thus conclude that the permissible period of warm ischemia before 3-month cryopreservation to maintain tracheal isograft viability is 18 hours in rats.






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