ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arinaga, M.
Right arrow Articles by Mitsudomi, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arinaga, M.
Right arrow Articles by Mitsudomi, T.
Related Collections
Right arrowRelated Article

Ann Thorac Surg 2000;70:401-405
© 2000 The Society of Thoracic Surgeons

Original articles: general thoracic

Expression of human telomerase subunit genes in primary lung cancer and its clinical significance

Mitsuyuki Arinaga, MDa, Shigeki Shimizu, MDa, Kunihiko Gotoh, MDa, Nobuhiro Haruki, MDb, Takao Takahashi, MDb, Takashi Takahashi, MDb, Tetsuya Mitsudomi, MDa

a Department of Department of Thoracic Surgery Aichi Cancer Center Hospital, , Aichi Cancer Center Research Institute, Aichi Cancer Center, Nagoya, Japan
b Department of Ultrastructure Research, Aichi Cancer Center Research Institute, Aichi Cancer Center, Nagoya, Japan

Address reprint requests to Dr Mitsudomi, Department of Thoracic Surgery, Aichi Cancer Center Hospital, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan
e-mail: mitsudom{at}leo.bekkoame.ne.jp

Background. Three major components of human telomerase, RNA component (hTERC), telomerase-associated protein (TEP1), and catalytic subunit (hTERT) have been cloned recently. The aim of this study was to examine the expression of these genes and to search for clinical usefulness.

Methods. Expression of these genes was evaluated by reverse transcription-polymerase chain reaction in 92 human lung cancers and in 32 non-neoplastic lung tissues. In 15 patients, both telomerase activity by telomeric repeat amplification protocol assay and expression were evaluated.

Results. hTERT expression was best associated with telomerase activity with a concordance of 77%. In 92 lung cancer tissues, hTERC, TEP1, and hTERT were expressed in 100%, 93%, and 89%, respectively. Whereas most adjacent non-neoplastic lung tissues expressed hTERC and TEP1 (94% and 100%, respectively), hTERT was detected in only 1 of 32 normal lungs. However, there was no relationship between hTERT expression and clinicopathologic features.

Conclusions. hTERT expression can be a surrogate for telomerase activity that may serve as a novel biomarker of lung cancer with high specificity and sensitivity.


Related Article

Invited commentary
David H. Harpole, Jr
Ann. Thorac. Surg. 2000 70: 405-406. [Extract] [Full Text] [PDF]



This article has been cited by other articles:


Home page
 ThoraxHome page
K M Fong, Y Sekido, A F Gazdar, and J D Minna
Lung cancer * 9: Molecular biology of lung cancer: clinical implications
Thorax, October 1, 2003; 58(10): 892 - 900.
[Abstract] [Full Text] [PDF]

Home page
 Eur. J. Cardiothorac. Surg.Home page
E. Dikmen, M. Kara, G. Dikmen, H. Cakmak, and P. Dogan
Detection of telomerase activity in bronchial lavage as an adjunct to cytological diagnosis in lung cancer
Eur. J. Cardiothorac. Surg., February 1, 2003; 23(2): 194 - 199.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
L. Wang, J.-C. Soria, B. L. Kemp, D. D. Liu, L. Mao, and F. R. Khuri
hTERT Expression Is a Prognostic Factor of Survival in Patients with Stage I Non-Small Cell Lung Cancer
Clin. Cancer Res., September 1, 2002; 8(9): 2883 - 2889.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2000 by The Society of Thoracic Surgeons.