ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
James D. St. Louis
Alan P. Kypson
Kevin P. Landolfo
James E. Lowe
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by St. Louis, J. D.
Right arrow Articles by Lowe, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by St. Louis, J. D.
Right arrow Articles by Lowe, J. E.

Ann Thorac Surg 2000;69:1351-1357
© 2000 The Society of Thoracic Surgeons

Original articles: Cardiovascular

An experimental model of chronic myocardial hibernation

James D. St. Louis, MDa, G. Chad Hughes, MDa, Alan P. Kypson, MDb, Timothy R. DeGrado, PhDc, Carolyn L. Donovan, MDb, R. Edward Coleman, MDc, Bangliang Yin, MDa, Charles Steenbergen, MD, PhDd, Kevin P. Landolfo, MDa, James E. Lowe, MDa

a Division of Cardiovascular and Thoracic Surgery, Duke University Medical Center, Durham, North Carolina, USA
b Division of Cardiology, Duke University Medical Center, Durham, North Carolina, USA
c Department of Radiology, Duke University Medical Center, Durham, North Carolina, USA
d Department of Pathology, Duke University Medical Center, Durham, North Carolina, USA

Address reprint requests to Dr Lowe, Department of Surgery, Duke University Medical Center, Box 3954, Durham, NC 27710
e-mail: lowe0004{at}mc.duke.edu

Background. Hibernating myocardium describes persistently impaired ventricular function at rest caused by reduced coronary blood flow. However, a realistic animal model reproducing this chronic ischemic state does not exist. The purpose of this study was to explore whether chronic low-flow hibernation could be produced in swine.

Methods. Miniswine underwent 90% stenosis of the left circumflex coronary artery. Positron emission tomography and dobutamine stress echocardiography were performed 3 and 30 days (n = 6) or 14 days (n = 4) after occlusion to evaluate myocardial blood flow and viability. Triphenyl tetrazolium chloride assessed percent infarction. Electron microscopy was used to identify cellular changes characteristic of hibernating myocardium.

Results. Positron emission tomography (13N-labeled-ammonia) 3 days after occlusion demonstrated a significant reduction in myocardial blood flow in the left circumflex distribution. This reduced flow was accompanied by increased glucose use (18F-fluorodeoxyglucose), which is consistent with hibernating myocardium. Thirty days after occlusion, positron emission tomography demonstrated persistent low flow with increased glucose use in the left circumflex distribution. Dobutamine stress echocardiography 3 days after occlusion demonstrated severe hypocontractility at rest in the left circumflex region. Regional wall motion improved with low-dose dobutamine followed by deterioration at higher doses (biphasic response), findings consistent with hibernating myocardium. The results of dobutamine stress echocardiography were unchanged 30 days after occlusion. Triphenyl tetrazolium chloride staining (n = 6) revealed a mean of 8% ± 2% infarction of the area-at-risk localized to the endocardial surface. Electron microscopy (n = 4) 14 days after occlusion demonstrated loss of contractile elements and large areas of glycogen accumulation within viable cardiomyocytes, also characteristic of hibernating myocardium.

Conclusions. Chronic low-flow myocardial hibernation can be reproduced in an animal model after partial coronary occlusion. This model may prove useful in the study of the mechanisms underlying hibernating myocardium and the use of therapies designed to improve blood flow to the heart.




This article has been cited by other articles:


Home page
 Cardiovasc ResHome page
R. Klocke, W. Tian, M. T. Kuhlmann, and S. Nikol
Surgical animal models of heart failure related to coronary heart disease
Cardiovasc Res, April 1, 2007; 74(1): 29 - 38.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
G. Heusch, R. Schulz, and S. H. Rahimtoola
Myocardial hibernation: a delicate balance
Am J Physiol Heart Circ Physiol, March 1, 2005; 288(3): H984 - H999.
[Abstract] [Full Text] [PDF]

Home page
 Eur Heart JHome page
S.R. Underwood, J. J Bax, J. v. Dahl, M. Y Henein, A. C van Rossum, E. R Schwarz, J.-L. Vanoverschelde, E. E.v. d. Wall, and W. Wijns
Imaging techniques for the assessment of myocardial hibernation: Report of a Study Group of the European Society of Cardiology
Eur. Heart J., May 2, 2004; 25(10): 815 - 836.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
G. C. Hughes, S. S. Biswas, B. Yin, R. E. Coleman, T. R. DeGrado, C. K Landolfo, J. E. Lowe, B. H. Annex, and K. P. Landolfo
Therapeutic angiogenesis in chronically ischemic porcine myocardium: comparative effects of bFGF and VEGF
Ann. Thorac. Surg., March 1, 2004; 77(3): 812 - 818.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
S. S. Biswas, G. C. Hughes, J. E. Scarborough, P. W. Domkowski, L. Diodato, M. L. Smith, C. Landolfo, J. E. Lowe, B. H. Annex, and K. P. Landolfo
Intramyocardial and intracoronary basic fibroblast growth factor in porcine hibernating myocardium: A comparative study
J. Thorac. Cardiovasc. Surg., January 1, 2004; 127(1): 34 - 43.
[Abstract] [Full Text] [PDF]

Home page
 SEMIN CARDIOTHORAC VASC ANESTHHome page
E. o. Cosar and C. J. O'Connor
Hibernation, Stunning, and Preconditioning: Historical Perspective, Current Concepts, Clinical Applications, and Future Implications
Seminars in Cardiothoracic and Vascular Anesthesia, June 1, 2003; 7(2): 115 - 140.
[Abstract] [PDF]

Home page
 J. Appl. Physiol.Home page
G. C. Hughes, M. J. Post, M. Simons, and B. H. Annex
Translational Physiology: Porcine models of human coronary artery disease: implications for preclinical trials of therapeutic angiogenesis
J Appl Physiol, May 1, 2003; 94(5): 1689 - 1701.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
R. A. Kloner and R. B. Jennings
Consequences of Brief Ischemia: Stunning, Preconditioning, and Their Clinical Implications: Part 2
Circulation, December 18, 2001; 104(25): 3158 - 3167.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
G.C. Hughes
Cellular models of hibernating myocardium: implications for future research
Cardiovasc Res, August 1, 2001; 51(2): 191 - 193.
[Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
G. C. Hughes, C. K. Landolfo, B. Yin, T. R. DeGrado, R. E. Coleman, K. P. Landolfo, and J. E. Lowe
Is chronically dysfunctional yet viable myocardium distal to a severe coronary stenosis hypoperfused?
Ann. Thorac. Surg., July 1, 2001; 72(1): 163 - 168.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
G. C. Hughes, A. P. Kypson, B. H. Annex, B. Yin, J. D. St. Louis, S. S. Biswas, R. E. Coleman, T. R. DeGrado, C. L. Donovan, K. P. Landolfo, et al.
Induction of angiogenesis after TMR: a comparison of holmium:YAG, CO2, and excimer lasers
Ann. Thorac. Surg., August 1, 2000; 70(2): 504 - 509.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
P. W. Domkowski, G. C. Hughes, and J. E. Lowe
Ameroid constrictor versus hydraulic occluder: creation of hibernating myocardium
Ann. Thorac. Surg., June 1, 2000; 69(6): 1984 - 1984.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2000 by The Society of Thoracic Surgeons.