ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by D’Ovidio, F.
Right arrow Articles by Patterson, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by D’Ovidio, F.
Right arrow Articles by Patterson, G. A.

Ann Thorac Surg 1999;68:1008-1013
© 1999 The Society of Thoracic Surgeons

Original Articles: General Thoracic

Endobronchial transfection of naked TGF-ß1 cDNA attenuates acute lung rejection

Franco D’Ovidio, MDa, Motoki Yano, MDa, Jon H. Ritter, MDb, Thalachallour Mohanakumar, PhDc, G. Alexander Patterson, MDa

a Division of Cardiothoracic Surgery, Washington University School of Medicine, St. Louis, Missouri, USA
b Department of Surgery, Washington University School of Medicine, St. Louis, Missouri, USA
c Department of Pathology, Washington University School of Medicine, St. Louis, Missouri, USA

Address reprint requests to Dr Patterson, Division of Cardiothoracic Surgery, Washington University School of Medicine, 3108 Queeny Tower, One Barnes-Jewish Hospital Plaza, St. Louis, MO 63110

Presented at the Thirty-fifth Annual Meeting of The Society of Thoracic Surgeons, San Antonio, TX, Jan 25–27, 1999.

Abstract

Background. We investigated endobronchial transfection of CAT and TGF-ß1 cDNA selectively delivered to the lung graft with or without liposomes.

Methods. Phase I: F344 rats received 130 µg of naked plasmid pCF1-CAT or complexed to liposome GL67 via left main bronchus instillation. Rats were awakened (pCF1-CAT, n = 4; GL67:pCF1-CAT, n = 4) or served as donors in an isogenic transplant (pCF1-CAT, n = 5; GL67:pCF1-CAT, n = 5). ELISA was performed on lungs, hearts, and livers on POD 2. Phase II: BN lungs received TGF-ß1 sense (n = 6); antisense (n = 5); GL67:TGF-ß1 sense (n = 10); or saline solution (n = 10). F344 recipients were sacrificed on POD 5. The arterial pO2 and rejection were assessed. RT-PCR for murine TGF-ß1 was performed.

Results. Phase I: CAT expression was 519 ± 287 pg and 63 ± 68 with pCF1-CAT and 104 ± 67 and 37 ± 45 with GL67:pCF1-CAT, respectively, in the non-transplant and in the transplant setting. No protein was detected in the hearts, livers, and in the native lung of the recipients. Phase II: RT-PCR confirmed murine TGF-ß1 transfection. pO2 was 362.7 ± 110.2 (mean mmHg ± SD) for sense TGF-ß1; 146.88 ± 85.5 for antisense; 241.5 ± 181.5 for GL67-TGF-ß1 sense; and 88.4 ± 38.7 for saline. TGF-ß1 sense versus all other groups, p < 0.05, GL67-TGF-ß1 sense versus saline, p = 0.01. Rejection was significantly lower for TGF-ß1 sense versus saline, p = 0.04.

Conclusions. Endobronchial administration of naked plasmid achieves selective transfection of lung grafts. Using this strategy, TGF-ß1 reduces early lung allograft rejection.




This article has been cited by other articles:


Home page
 Eur. J. Cardiothorac. Surg.Home page
K. Ohmori, S.-i. Takeda, S. Miyoshi, M. Minami, S. Nakane, M. Ohta, Y. Sawa, and H. Matsuda
Application of HVJ-liposome mediated gene transfer in lung transplantation--distribution and transfection efficiency in the lung
Eur. J. Cardiothorac. Surg., May 1, 2005; 27(5): 768 - 773.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
T. Suda, N. Daddi, T. Tagawa, S. A. Kanaan, B. D. Kozower, J. H. Ritter, and G. A. Patterson
Recipient intramuscular cotransfection of transforming growth factor {beta}1 and interleukin 10 ameliorates acute lung graft rejection
J. Thorac. Cardiovasc. Surg., April 1, 2005; 129(4): 926 - 931.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
T. Tagawa, B. D. Kozower, S. A. Kanaan, N. Daddi, T. Suda, T. Oka, and G. A. Patterson
Tumor necrosis factor inhibitor gene transfer ameliorates lung graft ischemia-reperfusion injury
J. Thorac. Cardiovasc. Surg., October 1, 2003; 126(4): 1147 - 1154.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
T. Suda, T. Tagawa, S. A. Kanaan, B. D. Kozower, N. Daddi, T. Mohanakumar, and G. A. Patterson
Adenovirus encoding soluble tumor necrosis factor {alpha} receptor immunoglobulin prolongs gene expression of a cotransfected reporter gene in rat lung
J. Thorac. Cardiovasc. Surg., October 1, 2003; 126(4): 1155 - 1161.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
S. A. Kanaan, B. D. Kozower, T. Suda, N. Daddi, T. Tagawa, J. H. Ritter, T. Mohanakumar, and G. A. Patterson
Intratracheal adenovirus-mediated gene transfer is optimal in experimental lung transplantation
J. Thorac. Cardiovasc. Surg., December 1, 2002; 124(6): 1130 - 1136.
[Abstract] [Full Text]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
T. Tagawa, T. Suda, N. Daddi, B. D. Kozower, S. A. Kanaan, T. Mohanakumar, and G. A. Patterson
Low-dose endobronchial gene transfer to ameliorate lung graft ischemia-reperfusion injury
J. Thorac. Cardiovasc. Surg., April 1, 2002; 123(4): 795 - 802.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
M. LIU, M. SUGA, A. A. MACLEAN, J. A. ST. GEORGE, D. W. SOUZA, and S. KESHAVJEE
Soluble Transforming Growth Factor-beta Type III Receptor Gene Transfection Inhibits Fibrous Airway Obliteration in a Rat Model of Bronchiolitis Obliterans
Am. J. Respir. Crit. Care Med., February 1, 2002; 165(3): 419 - 423.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
F. D'Ovidio, N. Daddi, T. Suda, K. Grapperhaus, and A. G. Patterson
Efficient naked plasmid cotransfection of lung grafts by extended lung/plasmid exposure time
Ann. Thorac. Surg., June 1, 2001; 71(6): 1817 - 1823.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
H. Itano, W. Zhang, J. H. Ritter, T. J. McCarthy, N. S. Yew, T. Mohanakumar, and G. A. Patterson
Endobronchial transfection of naked viral interleukin-10 gene in rat lung allotransplantation
Ann. Thorac. Surg., April 1, 2001; 71(4): 1126 - 1133.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
B. N. Mora, C. H. R. Boasquevisque, M. Boglione, J. M. Ritter, R. K. Scheule, N. S. Yew, L. Debruyne, L. Qin, J. S. Bromberg, and G. A. Patterson
Transforming growth factor-{beta}1 gene transfer ameliorates acute lung allograft rejection
J. Thorac. Cardiovasc. Surg., May 1, 2000; 119(5): 913 - 920.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
T. Suda, B. N. Mora, F. D’Ovidio, J. A. Cooper, M. Hiratsuka, W. Zhang, T. Mohanakumar, and G. A. Patterson
IN VIVO ADENOVIRUS-MEDIATED ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE TRANSFER AMELIORATES LUNG ALLOGRAFT ISCHEMIA-REPERFUSION INJURY
J. Thorac. Cardiovasc. Surg., February 1, 2000; 119(2): 297 - 304.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 1999 by The Society of Thoracic Surgeons.