HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Christopher G. A. McGregor Axel Haverich Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Strüber, M. Articles by Haverich, A. Search for Related Content PubMed PubMed Citation Articles by Strüber, M. Articles by Haverich, A.

Ann Thorac Surg 1997;63:1428-1435
© 1997 The Society of Thoracic Surgeons

---

Original Article: General Thoracic

Effects of Lung Preservation With Euro-Collins and University of Wisconsin Solutions on Endothelium-Dependent Relaxations

Department of Thoracic and Cardiovascular Surgery, Hannover Medical School, Hannover, Germany, and Department of Cardiovascular Surgery, and Physiology, Mayo Clinic and Mayo Foundation, Rochester, Minnesota

Accepted for publication November 16, 1996.

Background. This study compares the effect of lung preservation using flush perfusion of Euro-Collins or University of Wisconsin solution on the pulmonary vascular function of endothelium-dependent and endothelium-independent relaxations.

Methods. Rings of canine intrapulmonary arteries were studied after 6 hours of cold ischemia in Euro-Collins or University of Wisconsin preservation solution. Endothelium-dependent and endothelium-independent relaxations were induced in organ chamber experiments. To also study pulmonary resistance vessels, endothelium-dependent relaxations were induced in in vitro perfused intact rabbit lungs.

Results. In the organ chamber experiments, a moderate but significant (p < 0.05) reduction in endothelium-dependent relaxations were found in the perfused and stored vessels. In perfused rabbit lungs, a decrease in the endothelial response occurred immediately after perfusion with Euro-Collins solution. However, a recovery and overshooting response was found after preservation with either solution and 6 hours of cold ischemia. A significant increase in the sensitivity of smooth muscle cells to nitric oxide was shown in both preparations.

Conclusions. Both crystalloid perfusion fluids cause a decrease in endothelial function during the perfusion procedure. In contrast, endothelial function is well preserved during the ischemic time. University of Wisconsin solution induced a higher sensitivity of the vascular smooth muscle to the endothelium-derived relaxing factor nitric oxide. A reduction in pulmonary vascular resistance after University of Wisconsin preservation may be of importance in subsequent clinical lung transplantation.

This article has been cited by other articles:

				R.-Z. Zhang, Q. Yang, A. P. C. Yim, and G.-W. He Alteration of cellular electrophysiologic properties in porcine pulmonary microcirculation after preservation with University of Wisconsin and Euro-Collins solutions Ann. Thorac. Surg., June 1, 2004; 77(6): 1944 - 1950. [Abstract] [Full Text] [PDF]

				M. Struber, J. M. Hohlfeld, T. Kofidis, G. Warnecke, J. Niedermeyer, S. P. Sommer, and A. Haverich Surfactant function in lung transplantation after 24 hours of ischemia: Advantage of retrograde flush perfusion for preservation J. Thorac. Cardiovasc. Surg., January 1, 2002; 123(1): 98 - 103. [Abstract] [Full Text] [PDF]

				M. Struber, M. Wilhelmi, W. Harringer, J. Niedermeyer, M. Anssar, A. Kunsebeck, J. D. Schmitto, and A. Haverich Flush perfusion with low potassium dextran solution improves early graft function in clinical lung transplantation Eur. J. Cardiothorac. Surg., February 1, 2001; 19(2): 190 - 194. [Abstract] [Full Text] [PDF]

				M. Struber, J. M. Hohlfeld, S. Fraund, P. Kim, G. Warnecke, and A. Haverich Low-potassium dextran solution ameliorates reperfusion injury of the lung and protects surfactant function J. Thorac. Cardiovasc. Surg., September 1, 2000; 120(3): 566 - 572. [Abstract] [Full Text] [PDF]