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Ann Thorac Surg 1996;61:184-189
© 1996 The Society of Thoracic Surgeons

Original Articles: General Thoracic

Double-Lung Transplantation in the Rat: An Acute, Syngeneic In Situ Model

Bernard Hausen, MD, Stefanos Demertzis, MD, Frank Schröder, Maike Beuke, Hans-Joachim Schäfers, MD

Division of Thoracic and Cardiovascular Surgery, Surgical Center, Hannover Medical School, Hannover, Germany

Accepted for publication August 24, 1995.

Background. The high rate of reperfusion injury in clinical lung transplantation mandates significant improvements in lung preservation. Innovations should be validated using standardized and low-cost experimental models.

Methods. The model introduced here is analyzed by comparing global lung function after varying ischemic times (2, 4, 8, 16, and 24 hours). A rat double-lung block is flush-perfused, and the main pulmonary artery and left atrium are connected to the left pulmonary artery and vein of a syngeneic recipient using a T-shaped stent. With pressure side ports and incorporated flow crystals, measurement of vascular resistance and graft oxygenation can be performed. The transplant is ventilated separately, and compliance and resistance are determined.

Results. The increase in the ischemic interval from 2 to 24 hours caused an increase in the alveolar arterial oxygen difference from 220 ± 20 to 600 ± 34 mm Hg, pulmonary vascular resistance from 198 ± 76 to 638 ± 212 mm Hg•mL-1•min-1, and resistance to airflow from 274 ± 50 to 712 ± 30 cm H2O/L H2O, and a decrease in pulmonary compliance from 0.4 ± 0.05 to 0.12 ± 0.06 mL/cm H2O.

Conclusions. This in situ, syngeneic rat lung transplantation model offers an alternative to large animal models for verification of lung preservation solutions and for modification of donor or recipient treatment regimens.




This article has been cited by other articles:


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 Ann. Thorac. Surg.Home page
B. Hausen, P. Mueller, M. Bahra, R. Ramsamooj, R. E. Morris, and C. W. Hewitt
Donor Treatment With the Lazeroid U74389G Reduces Ischemia-Reperfusion Injury in a Rat Lung Transplant Model
Ann. Thorac. Surg., September 1, 1997; 64(3): 814 - 820.
[Abstract] [Full Text]

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 J. Thorac. Cardiovasc. Surg.Home page
B. Hausen, R. Rohde, C. W. Hewitt, F. Schroeder, M. Beuke, R. Ramsamooj, H.-J. Schafers, Sponsor:, and H.-G. Borst
EXOGENOUS SURFACTANT TREATMENT BEFORE AND AFTER SIXTEEN HOURS OF ISCHEMIA IN EXPERIMENTAL LUNG TRANSPLANTATION
J. Thorac. Cardiovasc. Surg., June 1, 1997; 113(6): 1050 - 1058.
[Abstract] [Full Text]


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