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Ann Thorac Surg 1995;60:1627-1632
© 1995 The Society of Thoracic Surgeons
a Department of Hematology-Oncology, Cornell University Medical Center, New York, New York, USA
b Divisions of Cardiothoracic Surgery and Circulatory Physiology, College of Physicians & Surgeons, Columbia University, New York, New York, USA
* Address reprint requests to Dr Oz, Department of Cardiothoracic Surgery, Columbia-Presbyterian Medical Center, MHB 7GN-435, 177 Fort Washington Ave, New York, NY 10032.
Background.: Textured biomaterial surfaces in implantable left ventricular assist devices induce development of a nonthrombotic neointimal surface and allow elimination of anticoagulation therapy in device recipients. Characterization of the hematopoietic cells formed within the neointimal surfaces of these devices will contribute to our understanding of this unique neointima.
Methods.: The blood-contacting surface of seven ThermoCardiosystems left ventricular assist devices was removed, washed with phosphate-buffered saline solution, and digested with 0.1% collagenase for 15 to 20 minutes. The hematopoietic cells released from the explants were isolated and analyzed by flow cytometry and immunohistochemical staining.
Results.: More than 80% ± 6% of hematopoietic cells isolated in this fashion are of myelomonocytic origin and express CD14, CD15, and CD33 surface molecules. Four percent of cells express the CD34 surface marker, which suggests that the neointima is colonized by pluripotent hematopoietic stem cells. Continuous culture of these hematopoietic cells in the presence of the cytokines interleukin-3, c-kit ligand, granulocyte colony—stimulating factor, and granulocyte/macrophage colony—stimulating factor resulted in tenfold expansion by day 7 and 25-fold expansion by day 14.
Conclusions.: Pluripotent hematopoietic cells with a high proliferative capacity colonize textured surfaces of left ventricular assist devices and may contribute to the development of a biologically nonthrombogenic neointima.
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