HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Paolo Macchiarini Guy-Michel Mazmanian Alain R. Chapelier Philippe G. Dartevelle Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Macchiarini, P. Articles by Dartevelle, P. G. Search for Related Content PubMed PubMed Citation Articles by Macchiarini, P. Articles by Dartevelle, P. G.

Ann Thorac Surg 1995;60:1597-1604
© 1995 The Society of Thoracic Surgeons

---

Original Articles: General Thoracic

Maximal Preservation Time of Tracheal Allografts

Department of Thoracic and Vascular Surgery and Heart-Lung Transplantation, Hôpital Marie-Lannelongue, Le Plessis Robinson, Paris-Sud University, Paris, France

Accepted for publication August 8, 1995.

Background. Our objective was to study the maximal preservation time of directly revascularized tracheal allografts in immunosuppressed piglets.

Methods. Donor grafts were flushed with Euro-Collins solution (65 mL/kg at 4°C) by simultaneous inferior thyroid artery and bronchial artery perfusion through a 15-cm aortic segment and heterotopically implanted on their own vascular pedicle after 3 (group 1), 6 (group 2), 15 (group 3), and 24 (group 4) hours of static storage in Euro-Collins solution at 4°C (n = 5 each). The animals were observed for 4 weeks after transplantation and then sacrificed. Histologic evaluation of the tracheal allografts was routinely done using specimens from open biopsies.

Results. The overall length of tracheal grafts was 12.4 ± 0.6 cm, and this variable was not significantly different between the four groups. Graft exocrine (mucous secretion) function began 1.3 ± 0.5 days after transplantation in groups 1 through 3 but was absent in all group 4 grafts (p < 0.0001). All grafts in groups 1 through 3 were viable at the time of sacrifice and showed little discernible intergroup and intragroup histologic and functional (tracheal smooth muscle contraction and relaxation) variations except for a significantly higher (p < 0.001) incidence of rejection in group 3 allografts. In contrast, all grafts in group 4 became completely necrotic 4 days after transplantation (p < 0.001) despite full patency of all the vascular anastomoses.

Conclusions. These results demonstrate that tracheal allografts may be safely preserved for as long as 15 hours and that longer periods of ischemia are likely to result in irreversible allograft damage.

This article has been cited by other articles:

				H. C. Grillo Tracheal replacement: a critical review Ann. Thorac. Surg., June 1, 2002; 73(6): 1995 - 2004. [Abstract] [Full Text] [PDF]

				K. Kushibe, T. Tojo, H. Sakaguchi, M. Takahama, K. Nishizaki, K. Nezu, and S. Taniguchi Effects of warm ischemia and cryopreservation on cartilage viability of tracheal allografts Ann. Thorac. Surg., December 1, 2000; 70(6): 1876 - 1879. [Abstract] [Full Text] [PDF]

				R. Nakanishi, M. Umesue, M. Hashimoto, H. Muranaka, M. Hachida, and K. Yasumoto Limit of warm ischemia time before cryopreservation in rat tracheal isografts Ann. Thorac. Surg., December 1, 2000; 70(6): 1880 - 1884. [Abstract] [Full Text] [PDF]

				P. Macchiarini, E. Dulmet, V. d. Montpreville, G.-M. Mazmanian, A. Chapelier, and P. Dartevelle TRACHEAL GROWTH AFTER SLIDE TRACHEOPLASTY J. Thorac. Cardiovasc. Surg., March 1, 1997; 113(3): 558 - 566. [Abstract] [Full Text]