ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Harvey I. Pass
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pass, H. I.
Right arrow Articles by Matthews, W. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pass, H. I.
Right arrow Articles by Matthews, W. J.
Related Collections
Right arrowRelated Article

Ann Thorac Surg 1995;59:835-844
© 1995 The Society of Thoracic Surgeons

Characteristics of Nine Newly Derived Mesothelioma Cell Lines

Harvey I. Pass, MD, Emily J. Stevens, MD, Herbert Oie, PhD, Maria G. Tsokos, MD, Andrea D. Abati, MD, Patricia A. Fetsch, MT(ASCP), Daphne J. Y. Mew, MD, PhD, Helen W. Pogrebniak, MD, Wilbert J. Matthews, BS

Surgery Branch, Thoracic Oncology Section; Naval Medical Oncology Branch; Pediatric Tumor Biology-Ultrastructural Pathology Section and Cytopathology Section, National Cancer Institute, National Institutes of Health, Bethesda, Maryland

This report characterizes nine new cell lines derived from patients with malignant pleural mesothelioma. The lines were initiated between July 1990 and July 1992 from solid tumors (5 lines) or effusions (4 lines) and had proliferated for a period of at least 2 months without senescence. They were characterized by cell size, doubling time, immunohistochemical analyses, electron microscopy, and chromosomal karyotyping. Growth factor/cytokine elaboration was determined using enzyme-linked immunoassays. The established lines were similar in morphology to their parent tumor (ie, epithelial or sarcomatoid). Cell sizes ranged from 59 to 81 µm, and the doubling times varied from 31 to 65 hours. The lines stained with cytokeratin and showed expected negative staining for adenomarkers including B72.3 and carcinoembryonic antigen. All cell lines exhibited aneuploidy, with modal chromosome numbers between 40 and 81 and had multiple chromosomal aberrations. Significant production of granulocyte--monocyte colony-stimulating factor, leukemia inhibitory factor, platelet-derived growth factor, and interleukin-6 was seen. These new cell lines derived from human mesotheliomas can now be used to aid in the design of innovative treatment strategies.


Related Article

DISCUSSION
Ann. Thorac. Surg. 1995 59: 844. [Extract] [Full Text]



This article has been cited by other articles:


Home page
 Clin. Cancer Res.Home page
T. Mukohara, G. Civiello, I. J. Davis, M. L. Taffaro, J. Christensen, D. E. Fisher, B. E. Johnson, and P. A. Janne
Inhibition of the Met Receptor in Mesothelioma
Clin. Cancer Res., November 15, 2005; 11(22): 8122 - 8130.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Stapelberg, N. Gellert, E. Swettenham, M. Tomasetti, P. K. Witting, A. Procopio, and J. Neuzil
{alpha}-Tocopheryl Succinate Inhibits Malignant Mesothelioma by Disrupting the Fibroblast Growth Factor Autocrine Loop: MECHANISM AND THE ROLE OF OXIDATIVE STRESS
J. Biol. Chem., July 8, 2005; 280(27): 25369 - 25376.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
C. D. Hoang, X. Zhang, P. D. Scott, T. J. Guillaume, M. A. Maddaus, D. Yee, and R. A. Kratzke
Selective Activation of Insulin Receptor Substrate-1 and -2 in Pleural Mesothelioma Cells: Association with Distinct Malignant Phenotypes
Cancer Res., October 15, 2004; 64(20): 7479 - 7485.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
C. D. Hoang, J. D'Cunha, M. G. Kratzke, C. E. Casmey, S. P. Frizelle, M. A. Maddaus, and R. A. Kratzke
Gene Expression Profiling Identifies Matriptase Overexpression in Malignant Mesothelioma
Chest, May 1, 2004; 125(5): 1843 - 1852.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. A. Janne, M. L. Taffaro, R. Salgia, and B. E. Johnson
Inhibition of Epidermal Growth Factor Receptor Signaling in Malignant Pleural Mesothelioma
Cancer Res., September 15, 2002; 62(18): 5242 - 5247.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. Toyooka, H. I. Pass, N. Shivapurkar, Y. Fukuyama, R. Maruyama, K. O. Toyooka, M. Gilcrease, A. Farinas, J. D. Minna, and A. F. Gazdar
Aberrant Methylation and Simian Virus 40 Tag Sequences in Malignant Mesothelioma
Cancer Res., August 1, 2001; 61(15): 5727 - 5730.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
S. C. Grondin and D. J. Sugarbaker
Pleuropneumonectomy in the Treatment of Malignant Pleural Mesothelioma*
Chest, December 1, 1999; 116(suppl_3): 450S - 454S.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
H. I. Pass, B. W. Robinson, J. R. Testa, and M. Carbone
Emerging Translational Therapies for Mesothelioma*
Chest, December 1, 1999; 116(suppl_3): 455S - 460S.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
J. B. RUBINS, T. GREATENS, R. A. KRATZKE, A. T. TAN, V. A. POLUNOVSKY, and P. BITTERMAN
Lovastatin Induces Apoptosis in Malignant Mesothelioma Cells
Am. J. Respir. Crit. Care Med., May 1, 1997; 157(5): 1616 - 1622.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 1995 by The Society of Thoracic Surgeons.