HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Blood, V. F. Articles by Mannion, J. D. Search for Related Content PubMed PubMed Citation Articles by Blood, V. F. Articles by Mannion, J. D.

The Annals of Thoracic Surgery, Vol 58, 1320-1325, Copyright © 1994 by The Society of Thoracic Surgeons

ARTICLES

Basic fibroblast growth factor identified in chronically stimulated cardiomyoplasties

VF Blood, MG Magno, WF Bailey, Y Shi, L Yurgenev, F DiMeo, RN Edie and JD Mannion
Department of Surgery, Jefferson Medical College, Philadelphia, Pennsylvania 19107.

In the presence of myocardial ischemia, chronic electrical stimulation of a latissimus dorsi (LD) cardiomyoplasty enhances extramyocardial collateral blood flow. We postulated that basic fibroblast growth factor (bFGF) may mediate extramyocardial collateral formation. To test this hypothesis, LDs from goats with cardiomyoplasties were probed for the presence of bFGF by Western blot analysis and immunohistochemistry. Three groups were studied: static LD cardiomyoplasty (group 1); LD cardiomyoplasty stimulated at a 2-Hz frequency for 6 weeks (group 2); and LD cardiomyoplasty electrically stimulated and given human recombinant bFGF (group 3). There was no evidence of bFGF in the left LDs of group 1 by Western blot. Basic fibroblast growth factor-like immunoreactive evidence was found in the left LDs of group 2 goats by both Western blot and immunohistochemistry. In the right LDs of group 2, bFGF-like material was found by immunohistochemistry but not by Western blot, which suggests that the tissue concentrations were low (near the limits of detection). The left LDs of group 3 were positive for bFGF by Western blot and immunohistochemistry. Group 3 right LDs were positive for bFGF by immunohistochemistry. Immunohistochemical findings in group 2 indicate that bFGF is present in goat skeletal muscle. Western blot data from groups 1 and 2 suggest that bFGF may be increased in chronically stimulated cardiomyoplasties. From findings in group 3, we conclude that exogenous bFGF does not downregulate, and may upregulate, endogenous production. These results support the possibility that skeletal muscle bFGF is an important factor in extramyocardial collateral formation.

This article has been cited by other articles:

				S. K. Gandhi, F. A. Pigula, and R. D. Siewers Successful late reintervention after the arterial switch procedure Ann. Thorac. Surg., January 1, 2002; 73(1): 88 - 95. [Abstract] [Full Text] [PDF]

				J.-W. Gu, D. Santiago, Y. Olowe, and J. Weinberger Basic Fibroblast Growth Factor as a Biochemical Marker of Exercise-Induced Ischemia Circulation, March 4, 1997; 95(5): 1165 - 1168. [Abstract] [Full Text]

				J. D. Mannion, V. Blood, W. Bailey, T. L. Bauer, M. G. Magno, F. DiMeo, A. Epple, and F. G. Spinale THE EFFECT OF BASIC FIBROBLAST GROWTH FACTOR ON THE BLOOD FLOW AND MORPHOLOGIC FEATURES OF A LATISSIMUS DORSI CARDIOMYOPLASTY J. Thorac. Cardiovasc. Surg., January 1, 1996; 111(1): 19 - 28. [Abstract] [Full Text]