HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Jill M. Siegfried Robert J. Keenan Rodney J. Landreneau Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Siegfried, J. M. Articles by Landreneau, R. J. Search for Related Content PubMed PubMed Citation Articles by Siegfried, J. M. Articles by Landreneau, R. J.

Ann Thorac Surg 1994;58:662-667
© 1994 The Society of Thoracic Surgeons

---

Articles

Ability to culture resectable non-small cell lung carcinomas is correlated with recurrence

^a Department of Pharmacology, Section of Thoracic Surgery, and Department of Pathology, University of Pittsburgh, Pittsburgh USA
^b Fox Chase Cancer Center, Philadelphia, Pennsylvania USA

^_* Address reprint requests to Dr Siegfried, Department of Pharmacology, E1347 Biomodical Science Tower, University of Pittsburgh, Pittsburgh, PA 15261.

Present clinicopathologic staging of non-small cell lung cancer is limited in its ability to provide more than a general prognostic estimate in patients with lung cancer who have resectable disease. This study was performed to identify whether the ability to adapt tumor tissue from resectable (stage I to IIIa) non-small cell lung cancer was associated with a poorer prognosis and an increased risk for early tumor recurrence. We attempted to culture a tumor specimen obtained from 90 patients with resectable non-small cell lung cancer. We used a culture medium conditioned by exposure to the lung cancer cell line A549-1, a known producer of autocrine lung cancer growth factors, and provided tumor colony scaffolding using a feeder layer of inactivated fibroblasts, and found these measures improved tumor culture yields. Twenty-two cell lines were obtained, a success rate of 24.4%. Tumor recurrences were more common (79%) among the culture-positive patients than among the culture-negative patients (37.5%; p < 0.002). For all patients, survival at 19 months in the culturepositive patients was 50.0%, compared with 83.6% in the culture-negative patients (p < 0.005). The median survival for the culture-positive patients was 15 months, versus 21.7 months for the culture-negative patients (p < 0.004). The establishment of a culture was a predictor of shortened survival for patients with stage I disease. In patients with stage I disease, survival at 19 months was 54.5% for the culture-positive patients, versus 89% for the culturenegative patients (p < 0.02). On the basis of our findings, the ability to adapt tumor specimens to cell culture appears to be a negative prognostic indicator for surgically resectable non-small cell lung cancer. The results of culture of the primary tumor may be used to more closely estimate the individual patient's risk for recurrence and determine his or her potential need for adjuvant therapies.

This article has been cited by other articles:

				R. J. Landreneau, D. J. Sugarbaker, M. J. Mack, S. R. Hazelrigg, J. D. Luketich, L. Fetterman, M. J. Liptay, S. Bartley, T. M. Boley, R. J. Keenan, et al. WEDGE RESECTION VERSUS LOBECTOMY for STAGE I (T1 N0 M0) NON-SMALL-CELL LUNG CANCER J. Thorac. Cardiovasc. Surg., April 1, 1997; 113(4): 691 - 700. [Abstract] [Full Text]