ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Ghannam A. Al-Dossari
Vibhu R. Kshettry
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Al-Dossari, G. A.
Right arrow Articles by Bolman, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Al-Dossari, G. A.
Right arrow Articles by Bolman, R. M., III

Ann Thorac Surg 1994;58:34-40
© 1994 The Society of Thoracic Surgeons

Articles

Experimental large-animal model of obliterative bronchiolitis after lung transplantation

Ghannam A. Al-Dossari, MD, Vibhu R. Kshettry, MD*, Jose Jessurun, MD, R.Morton Bolman, III, MD

Division of Cardiovascular and Thoracic Surgery, University of Minnesota, Minneapolis, Minnesota USA

* Address reprint requests to Dr Kshettry, Cardiovascular Surgery, University of Minnesota, 420 Delaware Si SE, Box 207 UMHC, Minneapolis, MN 55455-0392.

Obliterative bronchiolitis is a major cause of long-term morbidity after lung transplantation. It is characterized by small-airway inflammation and occlusion by fibrous tissue. The pathogenesis is uncertain. To study this disease, we developed a model of posttransplantation obliterative bronchiolitis using genetically defined miniature swine. Group 1 (n = 2) received a left lung autograft; group 2 (n = 7), a left lung allograft. Group 2 recipients were given cyclosporine, prednisone, and azathioprine for 3 months, then immunosuppression was tapered and discontinued over 1 month. The animals were observed for an additional 2 months, then sacrificed. Lung grafts in both groups were monitored with serial bronchoalveolar lavages and transbronchial biopsies for 6 months. After sacrifice, lung grafts underwent histopathologic and immunohistochemical examination. No allograft had histologic evidence of acute rejection or peribronchiolar infiltrate during the first 3 months of immunosuppression. During the tapering period, airway changes characterized by severe peribronchiolar lymphocytic infiltrates were seen. Bronchoalveolar lavages of allografts showed significantly increased lymphocyte counts with CD8+ cells predominating. After the discontinuation of immunosuppression, transbronchial biopsy and autopsy specimens showed progressive fibrous inflammatory occlusion of bronchioles. Immunohisto-chemical staining demonstrated increased expression of MCH class II antigen on the bronchiolar epithelium and increased dendritic cells and CD4+ lymphocytes. None of these changes were seen in group 1. Our findings suggest obliterative bronchiolitis is an immunologically mediated phenomenon related to chronic graft rejection after lung transplantation. This model will allow systematic study of the pathogenesis of obliterative bronchiolitis and possible therapeutic intervention.




This article has been cited by other articles:


Home page
 Lung Transplantation vol 26Home page
A. Boehler and M. Estenne
Post-transplant bronchiolitis obliterans
Lung Transplantation, July 1, 2010; 158 - 178.
[Abstract] [Fulltext] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
K. E. Scholz-Ahrens, G. Delling, B. Stampa, A. Helfenstein, H.-J. Hahne, Y. Acil, W. Timm, R. Barkmann, J. Hassenpflug, J. Schrezenmeir, et al.
Glucocorticosteroid-induced osteoporosis in adult primiparous Gottingen miniature pigs: effects on bone mineral and mineral metabolism
Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E385 - E395.
[Abstract] [Full Text] [PDF]

Home page
 Eur Respir JHome page
A. Boehler and M. Estenne
Post-transplant bronchiolitis obliterans
Eur. Respir. J., December 1, 2003; 22(6): 1007 - 1018.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. M. Aris, S. Walsh, W. Chalermskulrat, V. Hathwar, and I. P. Neuringer
Growth Factor Upregulation during Obliterative Bronchiolitis in the Mouse Model
, August 1, 2002; 166(3): 417 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
H. S. ALHO, K. A. INKINEN, U.-S. SALMINEN, P. K. MAASILTA, E. I. TASKINEN, V. GLUMOFF, E. I. VUORIO, T. S. IKONEN, and A. L. J. HARJULA
Collagens I and III in a Porcine Bronchial Model of Obliterative Bronchiolitis
, October 15, 2001; 164(8): 1519 - 1525.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
W. Y. Szeto, A. M. Krasinskas, D. Kreisel, S. H. Popma, and B. R. Rosengard
Donor antigen-presenting cells are important in the development of obliterative airway disease
J. Thorac. Cardiovasc. Surg., December 1, 2000; 120(6): 1070 - 1077.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
J.-P. Brouland, T. Egan, J. Roussi, M. Bonneau, G. Pignaud, C. Bal, M. Vaiman, P. Andre, P. Herve, G. M. Mazmanian, et al.
In Vivo Regulation of von Willebrand Factor Synthesis : von Willebrand Factor Production in Endothelial Cells After Lung Transplantation Between Normal Pigs and von Willebrand Factor-Deficient Pigs
Arterioscler Thromb Vasc Biol, December 1, 1999; 19(12): 3055 - 3062.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
R. ARENS, J. M. McDONOUGH, H. ZHAO, N. P. BLUMENTHAL, R. M. KOTLOFF, and M. M. GRUNSTEIN
Altered Lung Mechanics after Double-Lung Transplantation
, November 1, 1998; 158(5): 1403 - 1409.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
R. Morton Bolman III
Editorial: Intracoronary adenovirus-mediated transfer of immunosuppressive cytokine genes prolongs allograft survival
J. Thorac. Cardiovasc. Surg., April 1, 1998; 115(4): 819 - 821.
[Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
A. G. L. Lee, F. M. Wagner, M.-F. Chen, C. Serrick, A. Giaid, and H. Shennib
A novel charcoal-induced model of obliterative bronchiolitis-like lesions: Implications of chronic nonspecific airway inflammation in the development of posttransplantation obliterative bronchiolitis
J. Thorac. Cardiovasc. Surg., April 1, 1998; 115(4): 822 - 827.
[Abstract] [Full Text] [PDF]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
T. J. Kroshus, V. R. Kshettry, K. Savik, R. John, M. I. Hertz, and R. M. Bolman III
RISK FACTORS FOR THE DEVELOPMENT OF BRONCHIOLITIS OBLITERANS SYNDROME AFTER LUNG TRANSPLANTATION
J. Thorac. Cardiovasc. Surg., August 1, 1997; 114(2): 195 - 202.
[Abstract] [Full Text]

Home page
 Ann. Thorac. Surg.Home page
H. Reichenspurner, R. E. Girgis, R. C. Robbins, J. V. Conte, R. V. Nair, V. Valentine, G. J. Berry, R. E. Morris, J. Theodore, and B. A. Reitz
Obliterative bronchiolitis after lung and heart-lung transplantation
Ann. Thorac. Surg., December 1, 1995; 60(6): 1845 - 1853.
[Abstract] [PDF]

Home page
 Ann. Thorac. Surg.Home page
S. Sundaresan, E. P. Trulock, T. Mohanakumar, J. D. Cooper, G. A. Patterson, and The Washington University Lung Transplant Group
Prevalence and outcome of bronchiolitis obliterans syndrome after lung transplantation
Ann. Thorac. Surg., November 1, 1995; 60(5): 1341 - 1347.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 1994 by The Society of Thoracic Surgeons.