HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hoff, S. J. Articles by Sandler, M. P. Search for Related Content PubMed PubMed Citation Articles by Hoff, S. J. Articles by Sandler, M. P.

The Annals of Thoracic Surgery, Vol 56, 1074-1077, Copyright © 1993 by The Society of Thoracic Surgeons

ARTICLES

Noninvasive detection of acute rejection in a new experimental model of heart transplantation

SJ Hoff, JR Stewart, WH Frist, JB Atkinson, MW Kronenberg, J Votaw, RM Kessler and MP Sandler
Department of Cardiac and Thoracic Surgery and Radiology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-5734.

We have shown that positron emission scintigraphy detects changes in the uptake of 18-F 2-deoxyglucose and 13-N ammonia by the acutely rejecting myocardium in a nonworking model of heterotopic heart transplantation in the rat. We developed a new working model of heterotopic heart transplantation to determine the possible relevance of these changes to clinical transplantation. Moderate aortic valvular regurgitation was produced allowing the heterotopic left ventricle to fill and eject. Rejecting allografts and nonrejecting isografts (controls) were studied 4 days after transplantation. Histologically, isografts were normal and all allografts showed mild acute rejection. Decay-corrected uptakes of 18-F 2-deoxyglucose and 13-N ammonia reflect glucose metabolism and blood flow, respectively. Values are presented as percent of injected dose per gram of tissue. Uptake of 18-F 2- deoxyglucose was higher in rejecting allografts compared with nonrejecting isografts (3.0 +/- 1.8 versus 1.1 +/- 0.4; p = 0.024). Ammonia uptake was elevated in allografts compared with isografts (2.2 +/- 0.5 versus 1.3 +/- 0.5; p = 0.023). Uptakes of 18-F 2-deoxyglucose and 13-N ammonia are higher in mildly rejecting allografts, implying increased glucose utilization and blood flow during acute rejection. These data support our earlier findings of changes in myocardial metabolism in the absence of diminishing blood flow in acutely rejecting hearts. This model may lead to a better understanding of the physiology and metabolism of acute rejection.

This article has been cited by other articles:

				J. E. Everett, M. N. Palmer, J. Jessurun, and S. J. Shumway Noninvasive Diagnosis of Cardiac Allograft Rejection in an Orthotopic Canine Model Ann. Thorac. Surg., November 1, 1996; 62(5): 1337 - 1340. [Abstract] [Full Text]