HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Paul R.J. De Leyn Dirk E.M. Van Raemdonck Willem Flameng Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by De Leyn, P. R. J. Articles by Flameng, W. Search for Related Content PubMed PubMed Citation Articles by De Leyn, P. R. J. Articles by Flameng, W.

Ann Thorac Surg 1993;55:1073-1079
© 1993 The Society of Thoracic Surgeons

---

Articles

Effect of inflation on adenosine triphosphate catabolism and lactate production during normothermic lung ischemia

Division of Thoracic Surgery and Center of Experimental Surgery and Anesthesiology, Katholieke Universiteit Leuven, Leuven, Belgium

^_* Address reprint requests to Dr De Leyn, Center of Experimental Surgery and Anesthesiology, Minderbroederstraat 17, Provisorium 1, B-3000 Leuven, Belgium.

Although few biochemical data comparing adenosine triphosphate (ATP) catabolism or lactate production in isolated deflated versus inflated lung tissue are available, most transplant centers preserve their donor lungs inflated. We measured ATP level (using high-performance liquid chromatography), energy charge, and lactate level during 2 hours of normothermic ischemia in deflated lung tissue (n = 6), in lung tissue inflated with room air (n = 6), and in lung tissue inflated with 100% oxygen (n = 6). To determine the onset of anaerobic metabolism in lung issue inflated with 100% O₂, ATP and lactate levels were measured in another group (n = 6) during 8 hours of normothermic ischemia. Rabbit lungs were flushed in situ with a modified Krebs-Henseleit solution (60 mL/kg). They were isolated and immersed in 0.9% Nacl at 37 °C. In deflated lung tissue, ATP level (control value, 9.4 ± 0.58 µmol/g dry wt) decreased and lactate level (control value, 5.6 ± 1.16 µmol/g dry wt) increased after 15 minutes of ischemia (ATP, 5.2 ± 0.86 µmol/g dry wt; lactate, 13.3 ± 1.58 µmol/g dry wt). When the lung was stored inflated with room air, ATP breakdown and increase of lactate concentration only occurred after 90 minutes of normothermic ischemia (at 60 minutes: ATP, 8.0 ± 0.58 µmol/g dry wt; lactate, 6.3 ± 1.1 µmol/g dry wt). In lungs stored inflated with 100% O₂, ATP breakdown and lactate accumulation only occurred after 5 hours of normothermic ischemia (at 4 hours: ATP, 8.1 ± 0.74 µmol/g dry wt; lactate, 5.9 ± 1.28 µmol/g dry wt). These data suggest that the breakdown of ATP and the onset of anaerobic metabolism in lung tissue is related to the alveolar oxygen concentration during storage, and that in the isolated inflated lung, aerobic metabolism continues for 4 hours even when perfusion has stopped.

This article has been cited by other articles:

				H. Imura, M. Caputo, K. Lim, M. Ochi, M.-S. Suleiman, K. Shimizu, and G. D. Angelini Pulmonary injury after cardiopulmonary bypass: Beneficial effects of low-frequency mechanical ventilation. J. Thorac. Cardiovasc. Surg., June 1, 2009; 137(6): 1530 - 1537. [Abstract] [Full Text] [PDF]

				F. R. Rega, A. P. Neyrinck, G. M. Verleden, T. E. Lerut, and D. E. M. Van Raemdonck How long can we preserve the pulmonary graft inside the nonheart-beating donor? Ann. Thorac. Surg., February 1, 2004; 77(2): 438 - 444. [Abstract] [Full Text] [PDF]

				S. V. Baudouin Lung injury after thoracotomy Br. J. Anaesth., July 1, 2003; 91(1): 132 - 142. [Full Text] [PDF]

				F. R Rega, E. J Vandezande, N. C Jannis, G. M Verleden, T. E Lerut, and D. E. Van Raemdonck The role of leukocyte depletion in ex vivo evaluation of pulmonary grafts from (non-)heart-beating donors Perfusion, January 1, 2003; 18(1_suppl): 13 - 21. [Abstract] [PDF]

				T. FUKUSE, T. HIRATA, T. NAKAMURA, M. KAWASHIMA, S. HITOMI, and H. WADA Influence of Deflated and Anaerobic Conditions During Cold Storage on Rat Lungs , August 1, 1999; 160(2): 621 - 627. [Abstract] [Full Text] [PDF]

				T. Sakuma, K. Takahashi, N. Ohya, O. Kajikawa, T. R. Martin, K. H. Albertine, and M. A. Matthay Ischemia-reperfusion lung injury in rabbits: mechanisms of injury and protection Am J Physiol Lung Cell Mol Physiol, January 1, 1999; 276(1): L137 - L145. [Abstract] [Full Text] [PDF]

				S. Murakami, E. A. Bacha, P. Herve, H. Detruit, A. R. Chapelier, P. G. Dartevelle, and G.-M. Mazmanian Prevention of Reperfusion Injury by Inhaled Nitric Oxide in Lungs Harvested From Non-Heart-Beating Donors Ann. Thorac. Surg., December 1, 1996; 62(6): 1632 - 1638. [Abstract] [Full Text]

				D. E. M. Van Raemdonck, N. C. P. Jannis, F. R. L. Rega, P. R. J. De Leyn, W. J. Flameng, and T. E. Lerut Delay of Adenosine Triphosphate Depletion and Hypoxanthine Formation in Rabbit Lung After Death Ann. Thorac. Surg., July 1, 1996; 62(1): 233 - 240. [Abstract] [Full Text]

				L. Normandin, P. Herve, C. Brink, A. R. Chapelier, P. G. Dartevelle, G.-M. Mazmanian, and Paris-Sud University Lung Transplant Group L-arginine and pentoxifylline attenuate endothelial dysfunction after lung reperfusion injury in the rabbit Ann. Thorac. Surg., September 1, 1995; 60(3): 646 - 650. [Abstract] [PDF]