ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
 QUICK SEARCH:   [advanced]


     


This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Patrick W. Domkowski
Robert H. Messier
Donald G. Crescenzo
Anwar S. Abd-Elfattah
Robert B. Wallace
Richard A. Hopkins
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Domkowski, P. W.
Right arrow Articles by Hopkins, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Domkowski, P. W.
Right arrow Articles by Hopkins, R. A.

Ann Thorac Surg 1993;55:413-419
© 1993 The Society of Thoracic Surgeons


Articles

Preimplantation alteration of adenine nucleotides in cryopreserved heart valves

Patrick W. Domkowski, MSa,b,c, Robert H. Messier, MDa,b,c, Donald G. Crescenzo, MDa,b,c, Hamdy S. Aly, MD, PhDa,b,c, Anwar S. Abd-Elfattah, PhDa,b,c, Stephen L. Hilbert, PhDa,b,c, Robert B. Wallace, MDa,b,c, Richard A. Hopkins, MD*,a,b,c

a Georgetown University School of Medicine, Washington, DC USA
b Medical College of Virginia, Richmond, Virginia USA
c Food and Drug Administration, Rockville, Maryland USA

Accepted for publication May 18, 1992.

* Address reprint requests to Dr Hopkins, Department of Surgery, 4PHC, Georgetown University, 3800 Reservoir Rd, NW, Washington, DC 20007.

To assess the initial metabolic phase of cellular injury from cardiac valve processing, high-energy phosphate concentrations were analyzed in valve leaflets subsequent to critical processing steps. Using a porcine model, valves were processed in a manner identical to human homografts, with 58 randomly assigned to five groups representing distinct preparation phases. Group I (controls) sustained 40 minutes of warm ischemia concluded by liquid nitrogen immersion. Remaining groups similarly endured 40 minutes of ischemia, but were subsequently prepared according to stepwise design: II, warm ischemia + 24 hours of 4 °C ischemia; III, warm ischemia + 24 hours of 4 °C antibiotic disinfection; IV, warm ischemia + 24 hours at 4 °C (without antibiotics) + cryopreservation (–1 °C/min cryoprotected freezing); and V, warm ischemia + disinfection + cryopreservation. At each regimen's conclusion leaflet extracts were assayed by high-performance liquid chromatography for high-energy adenine nucleotides (adenosine triphosphate, adenosine diphosphate, adenosine monophosphate) and catabolites. A 47% and 86% decrease in cellular adenosine triphosphate level was observed in group III and group V leaflets, respectively. The level of total adenine nucleotides was maintained up to cryopreservation; thereafter a 74% decrease was noted. Catabolite analysis confirmed incomplete degradation of adenine nudeotides indicating cellular metabolic resilience throughout standard homograft preparation in valves previously exposed to 40 minutes of warm ischemia.




This article has been cited by other articles:


Home page
Ann. Thorac. Surg.Home page
H. J. Geissler, U. M. Fischer, S. Foerster, A. Krahwinkel, A. Antonyan, A. Kroener, K. Addicks, U. Mehlhorn, and W. Bloch
Effect of Thawing on Nitric Oxide Synthase III and Apoptotic Markers in Cryopreserved Human Allografts
Ann. Thorac. Surg., November 1, 2006; 82(5): 1742 - 1746.
[Abstract] [Full Text] [PDF]


Home page
Ann. Thorac. Surg.Home page
E. Raanani, T. M. Yau, T. E. David, G. Dellgren, B. D. Sonnenberg, and A. Omran
Risk factors for late pulmonary homograft stenosis after the Ross procedure
Ann. Thorac. Surg., December 1, 2000; 70(6): 1953 - 1957.
[Abstract] [Full Text] [PDF]


Home page
J. Thorac. Cardiovasc. Surg.Home page
R. H. Messier Jr, B. L. Bass, P. W. Domkowski, and R. A. Hopkins
INTERSTITIAL CELLULAR AND MATRIX RESTORATION OF CARDIAC VALVES AFTERCRYOPRESERVATION
J. Thorac. Cardiovasc. Surg., July 1, 1999; 118(1): 36 - 49.
[Abstract] [Full Text] [PDF]


Home page
J. Thorac. Cardiovasc. Surg.Home page
S. L. Hilbert, R. E. Luna, J. Zhang, Y. Wang, R. A. Hopkins, Z.-X. Yu, and V. J. Ferrans
ALLOGRAFT HEART VALVES: THE ROLE OF APOPTOSIS-MEDIATED CELL LOSS
J. Thorac. Cardiovasc. Surg., March 1, 1999; 117(3): 454 - 462.
[Abstract] [Full Text] [PDF]


Home page
J. Thorac. Cardiovasc. Surg.Home page
J. P. Neves, S. Gulbenkian, T. Ramos, A. P. Martins, M. C. Caldas, R. Mascarenhas, M. Guerreiro, A. Matoso-Ferreira, R. Santos, C. Monteiro, et al.
MECHANISMS UNDERLYING DEGENERATION OF CRYOPRESERVED VASCULAR HOMOGRAFTS
J. Thorac. Cardiovasc. Surg., June 1, 1997; 113(6): 1014 - 1021.
[Abstract] [Full Text]


Home page
J. Thorac. Cardiovasc. Surg.Home page
C. Wassenaar, W. A. Bax, R.-J. van Suylen, V. D. Vuzevski, and E. Bos
EFFECTS OF CRYOPRESERVATION ON CONTRACTILE PROPERTIES OF PORCINE ISOLATED AORTIC VALVE LEAFLETS AND AORTIC WALL
J. Thorac. Cardiovasc. Surg., January 1, 1997; 113(1): 165 - 172.
[Abstract] [Full Text]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 1993 by The Society of Thoracic Surgeons.