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Ann Thorac Surg 1992;54:915-920
© 1992 The Society of Thoracic Surgeons

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Articles

Isolated biventricular working rat heart preparation

Division of Thoracic Surgery, Department of Surgery, Allegheny Campus of the Medical of Pennsylvania, and Allegheny singer Research Institute, Pittsburgh, Pennsylvania, USA

Accepted for publication March 5, 1992.

^_* Address reprint requests to Dr Kao, Department of Surgery, East Tennessee State University, Box 70575, Johnson City, TN 37614-0575.

The isolated perfused heart from small animals has been used extensively for hemodynamic and metabolic studies. The left working heart preparation proved superior to the Langendorff model for functional evaluations but has not allowed study of right heart function. A simple and inexpensive biventricular working heart preparation has been developed by modifying the left working rat heart model. Under general anesthesia the heart was removed surgically leaving sufficient vessels attacked to it. Cannulation of the aorta, left atrium, right atrium, and pulmonary artery was completed in 10 minutes. A pressurized compliance chamber allowed rapid and reliable regulation of aortic impedance. For the 7 hearts that were subjected to 3-hour biventricular perfusion (their end points expressed as percent of their initial values), the aortic output (95% ± 3%), pulmonary flow (88% ± 9%), mean aortic pressure (109% ± 5%), mean pulmonary pressure (100% ± 2%), heart rate (106% ± 8%), myocardial adenosine triphosphate level (85% ± 8%), and creatine phosphate level (89% ± 4%) were all maintained at physiologic levels. For the 11 hearts that were converted from left working heart preparation to biventricular working mode, significant improvement in stroke volume, aortic and cardiac output, and pressure development were observed. Experimental results indicate that the biventricular working model for isolated perfused rat hearts is superior to the left working preparation for studying the function of the total heart. Further study of the biventricular perfused working rat heart appears warranted.

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