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Ann Thorac Surg 1992;53:871-874
© 1992 The Society of Thoracic Surgeons

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Articles

A surgical technique that preserves human saphenous vein functional integrity

^a Department of Cardiac Surgery, The University of Sheffield, Sheffield, United Kingdom
^b Departments of Cardiac Surgery and Cardiology, University of Wales College of Medicine, Cardiff, United Kingdom

Accepted for publication October 29, 1991.

^_* Address reprint requests to Mr Angelini, Department of Cardiac Surgery, The University of Sheffield, Clinical Sciences Centre, Northern General Hospital, Sheffield 55 7AU, United Kingdom.

We investigated the effect of a novel surgical preparative technique for human saphenous vein by using the concentration of adenosine triphosphate and the adenosine triphosphate/diphosphate ratio to quantify medial integrity and by using stimulated rates of prostacyclin production to quantify endothelial function. Freshly isolated vein had an adenosine triphosphate concentration of 358 ± 54 nmol · g^–1 wet weight and an adenosine triphosphate/diphosphate ratio of 2.89 ± 0.13 (n = 12); it produced prostacyclin in response to fluid shear at a rate of 14.3 ± 2.0 pg · min^–1 · mg^–1 wet weight (n = 12). Surgically prepared vein obtained on completion of the last proximal anastomosis had been distended with the patient's own arterial pressure using a side-arm connected to the aortic cannula. This vein had an adenosine triphosphate concentration of 413 ± 70 nmol · g^–1 wet weight and an adenosine triphosphate/diphosphate ratio of 2.74 ± 0.44 (n = 11), and it produced prostacyclin at a rate of 13.1 ± 0.2 pg · min^–1 · mg^–1 wet weight (n = 12). All values were indistinguishable from those in freshly isolated vein. The results demonstrate that this simple technique for distention at arterial pressure preserved medial and endothelial function.

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