HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Dao M. Nguyen David S. Mulder Hani Shennib Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Nguyen, D. M. Articles by Shennib, H. Search for Related Content PubMed PubMed Citation Articles by Nguyen, D. M. Articles by Shennib, H.

Ann Thorac Surg 1992;53:611-616
© 1992 The Society of Thoracic Surgeons

---

Articles

Effect of cardiopulmonary bypass on circulating lymphocyte function

Montreal Lung Transplant Program and The Montreal General Hospital, Montreal, Quebec, Canada

Received for publication September 6, 1991. ^_* Address reprint requests to Dr Shennib, The Montreal General Hospital, 1650 Cedar Ave, Room 9827 L.H., Montreal, Que, Canada H3G 1A4.

Extracorporeal cardiopulmonary bypass (CPB) has been associated with a wide variety of immunalogical derangements, including a transient postoperative impairment of lymphocyte function. We examined changes in phenotypic and nonspecific cytotoxicity of peripheral blood mononuclear cells after extracorporeal CPB. The peripheral blood samples obtained from 10 patients were subjected to natural killer and cytotoxic T lymphocyte activity assay before and at intervals after CPB. Phenotypic] analysis of peripheral blood lymphocytes was performed in 5 patients before and immediately after CPB. We observed a significant increase in peripheral blood CD8+ cells (cytotoxic/suppressor T lymphocytes) (16.1% ± 2.5% versus 22.5% ± 2.1%; p < .005) and a decrease in CD4+ cells (helper/inducer T lymphocytes) (46.1% ± 3.5% versus 36.1% ± 3.5%;p < 0.02) immediately after extracorporeal circulation. The CD8/CD4 ratio in peripheral blood was significantly increased immediately after bypass (0.53 versus 0.80; p < 9.001). No significant changes in percentages of other leukocyte subsets in peripheral blood were noted. The activity of cytotoxic T lymphocytes and natural killer cells in peripheral blood was impaired on postoperative days 1 and 3 but was restored to preoperative values by removal of mononuclear phagocytes from these cells. The decrease in natural killer cell and cytotoxic T lymphocyte activity in peripheral blood may signify a temporary impairment of the effector arm of the cell-mediated immunity in the post-operative period. The observed changes in peripheral blood phenotype and function may be involved in early organ injury and infectious complications after CPB.

This article has been cited by other articles:

				T. Sano, S. Morita, M. Masuda, Y. Tomita, T. Nishida, H. Tatewaki, and H. Yasui Cardiopulmonary bypass, steroid administration, and surgical injury synergistically impair memory T cell function and antigen presentation Interact CardioVasc Thorac Surg, December 1, 2003; 2(4): 598 - 602. [Abstract] [Full Text] [PDF]

				H. Masuda, Y. Moriyama, R. Toda, S. Shimokawa, Y. Iguro, S.-i. Watanabe, K. Hisatomi, H. Matsumoto, and A. Taira The influence of human T lymphotropic virus type I infection on the outcome of cardiovascular surgery J. Thorac. Cardiovasc. Surg., October 1, 2000; 120(4): 699 - 706. [Abstract] [Full Text] [PDF]

				J. Katoh, K. Tsuchiya, H. Osawa, W. Sato, G. Matsumura, Y. Iida, S. Suzuki, S. Hosaka, S. Yoshii, and Y. Tada Cimetidine reduces impairment of cellular immunity after cardiac operations with cardiopulmonary bypass J. Thorac. Cardiovasc. Surg., August 1, 1998; 116(2): 312 - 316. [Abstract] [Full Text] [PDF]

				K. Kawahito, E. Kobayashi, Y. Misawa, H. Adachi, A. Fujimura, T. Ino, and K. Fuse Recovery From Lymphocytopenia and Prognosis After Adult Extracorporeal Membrane Oxygenation Arch Surg, February 1, 1998; 133(2): 216 - 217. [Abstract] [Full Text] [PDF]

				K. Hisatomi, A. Kobayashi, Y. Moriyama, S. Shimokawa, H. Toyohira, and A. Taira COMBINED SUPPRESSIVE EFFECT OF CARDIOPULMONARY BYPASS AND AGING ON CELL-MEDIATED IMMUNITY J. Thorac. Cardiovasc. Surg., July 1, 1997; 114(1): 140 - 141. [Full Text]

				P. Hornick Cardiopulmonary bypass and the adaptive immune system: perspectives on T cell function Perfusion, May 1, 1996; 11(3): 281 - 290. [PDF]

				D. M. Nguyen, D. S. Mulder, and H. Shennib Natural killer cell activity after cardiopulmonary bypass Ann. Thorac. Surg., July 1, 1994; 58(1): 274 - 275. [PDF]