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Ryo Aeba
Robert J. Keenan
Robert L. Hardesty
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Ann Thorac Surg 1992;53:240-246
© 1992 The Society of Thoracic Surgeons

Articles

University of Wisconsin solution for pulmonary preservation in a rat transplant model

Ryo Aeba, Md1, Robert J. Keenan, MD, Robert L. Hardesty, MD, Samuel A. Yousem, MD, Isao Hamamoto, MD, Bartley P. Griffith, MD*

Departments of Surgery and Pathology, University of Pittsburgh, Pittsburgh, Pennsylvania, USA

* Address reprint requests to Dr Griffth, Division of Cardiothoracic Surgery, University of Pittsburgh, Room 1084 Scaife Hall, Pittsburgh, PA 15261.

University of Wisconsin and modified Euro-Collins solutions for pulmonary preservation were compared in a rat orthotopic left lung isotransplant model. Heart-lung blocks of donor rats were flushed with and preserved in one of the preservation solutions at 0 °C. After 6 or 12 hours of cold ischemia, the left lungs were transplanted into recipient rats and repcrfused for 1 hour. Pulmonary function was assessed by measuring oxygen and carbon dioxide tensions in arterial blood after removal of the right lung. Lipid peroxide concentrations were measured as thiobarbiturate acid-reactive substances. The ratios of wet to dry weight of grafts after ischemia and after reperfusion were calculated. Histologic changes of ischemia-reperfusion injury of the lung tissue were evaluated using a graded scale. Oxygen tension after 6 hours of preservation followed by reperfusion was significantly higher with University of Wisconsin solution (308.8 ± 81.1 mm Hg) than with Euro-Collins solution (50.8 ± 17.8 mm Hg; p < 0.001). Carbon dioxide tension in the University of Wisconsin solution group was also significantly lower than in the Euro-Collins solution group (28.2 ± 2.3 versus 46.0 ± 4.5 mm Hg; p < 0.05). Lipid peroxide concentration after 6 hours' preservation in University of Wisconsin solution was significantly lower (0.88 ± 0.07 µmol/g) than that in Euro-Collins solution (1.26 ± 0.12 µmol/g; p < 0.05). After 12 hours of preservation only lipid peroxide concentration with University of Wisconsin solution was significantly lower (1.30 ± 0.09 µmol/g) than with Euro-Collins solution (1.71 ± 0.15 µmol/g; p < 0.05). There were no differences in the other variables. We conclude that University of Wisconsin solution provides superior lung preservation than does Euro-Collins solution in this transplant model.




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