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Ann Thorac Surg 1991;52:987-992
© 1991 The Society of Thoracic Surgeons

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Articles

Isolated, perfused neontal rat heart preparation for studies of calcium and functional stability

^a Cardiovascular Research, The Rayne Institute, St Thomas' Hospital, London, England
^b E.R. is a Visiting International Research Fellow from Istituto Ricerche Farmacologiche "Mario Negri" Via Eritrea 62, 20157 Milan, Italy

^_* Address reprint requests to Dr Riva, Istituto Ricerche Farmacologiche "Mario Negri," Via Eritrea 62, 20157 Milan, Italy.

We describe an isolated, perfused preparation for neonatal rat hearts to assess the relationship between extracellular calcium and (1) cardiac function and (2) contractile stability over 2 hours of perfusion. Neonatal (3 to 5 days old) rat hearts (n = 6 per group) were perfused for 30 minutes (Langendorff) with oxygenated buffer (37 °C) containing 1.4 mmol/L calcium (control period) and 90 minutes with buffer containing 0.5, 0.8, 1.0, 1.2, 1.4, 1.8, or 2.5 mmol/L calcium. Upon changing from 1.4 mmol/L to either a higher or low calcium concentration there were no significant changes in left ventricular developed pressure, heart rate, or coronary flow. However, left ventricular developed pressure progressively deteriorated in a time-dependent and calcium-dependent manner. Thus, after 90 minutes, developed pressure fell to 18% ± 2%, 27% ± 3%, 41% ± 5%, 47% ± 8%, 55% ± 10%, 64% ± 5% and 76% ± 4% of its initial value with 0.5, 0.8, 1.0, 1.2, 1.4, 1.8, and 2.5 mmol/L calcium. In conclusion, in studies with the neonatal rat heart, extracellular calcium concentrations in the range of 1.8 to 2.5 mmol/L are recommended.

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