HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kato, Y. Articles by Imamura, H. Search for Related Content PubMed PubMed Citation Articles by Kato, Y. Articles by Imamura, H.

The Annals of Thoracic Surgery, Vol 52, 979-986, Copyright © 1991 by The Society of Thoracic Surgeons

ARTICLES

Effect of cardioplegic preservation on intracellular calcium transients

Y Kato, H Otani, K Tanaka, Y Saito, M Fukunaka and H Imamura
Department of Thoracic and Cardiovascular Surgery, Kansai Medical University, Osaka, Japan.

Intracellular Ca2+ ([Ca2+]i) plays a pivotal role in the regulation of cardiac function. We investigated the effect of cardioplegic preservation on [Ca2+]i transients in the isolated and perfused guinea pig heart loaded with a fluorescent Ca2+ indicator (fura-2). The measurements of [Ca2+]i transients and isovolumetric left ventricular pressure revealed that [Ca2+]i transients and mechanical responses to [Ca2+]i were markedly altered during 15 minutes of normothermic global ischemia and after reperfusion. First, [Ca2+]i transients remained during ischemia without generation of active force of contraction. Such a manifestation of depressed sensitivity of the myofilaments to Ca2+ persisted for the first 5 minutes after reperfusion. Second, the amplitude of [Ca2+]i was diminished during ischemia and reperfusion. Third, diastolic [Ca2+]i was increased during ischemia and especially at the onset of reperfusion. Bolus infusion of cold St. Thomas' Hospital solution abolished [Ca2+]i transients and left ventricular pressure development at an end-diastolic level. Moreover, improved recovery of left ventricular function during reperfusion afforded by the hypothermic cardioplegia was intimately related to its ability to modulate impaired [Ca2+]i transients and mechanical responses to [Ca2+]i; improvement of systolic left ventricular function appears to be produced by restoration of Ca2+ sensitivity of the myofilaments and the amplitude of [Ca2+]i transients, whereas better diastolic compliance of the left ventricle is ascribed to significantly lower diastolic [Ca2+]i. These results may provide new insight into the mechanism of cardioplegic preservation on the basis of [Ca2+]i transients.

This article has been cited by other articles:

				K. Lu, H. Otani, T. Yamamura, Y. Nakao, R. Hattori, H. Ninomiya, M. Osako, and H. Imamura Protein kinase C isoform-dependent myocardial protection by ischemic preconditioning and potassium cardioplegia J. Thorac. Cardiovasc. Surg., January 1, 2001; 121(1): 0137 - 148. [Abstract] [Full Text] [PDF]

				B. H. Dorman, L. Hebbar, R. B. Hinton, R. C. Roy, and F. G. Spinale Preservation of Myocyte Contractile Function After Hypothermic Cardioplegic Arrest by Activation of ATP-Sensitive Potassium Channels Circulation, October 7, 1997; 96(7): 2376 - 2384. [Abstract] [Full Text]

				B. H. Dorman, M. J. Cavallo, R. B. Hinton, R. C. Roy, and F. G. Spinale PRESERVATION OF MYOCYTE CONTRACTILE FUNCTION AFTER HYPOTHERMIC, HYPERKALEMIC CARDIOPLEGIC ARREST WITH 2,3-BUTANEDIONE MONOXIME J. Thorac. Cardiovasc. Surg., March 1, 1996; 111(3): 621 - 629. [Abstract] [Full Text]

				T. Ko, H. Otani, H. Imamura, K. Omori, and C. Inagaki Role of sodium pump activity in warm induction of cardioplegia combined with reperfusion of oxygenated cardioplegic solution J. Thorac. Cardiovasc. Surg., July 1, 1995; 110(1): 103 - 110. [Abstract] [Full Text]

				T. K. Steigen, E. Aasum, T. Myrmel, and T. S. Larsen Effects of fatty acids on myocardial calcium control during hypothermic perfusion J. Thorac. Cardiovasc. Surg., January 1, 1994; 107(1): 233 - 241. [Abstract] [Full Text]