ATS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow References
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Truls Myrmel
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mjøs, O. D.
Right arrow Articles by Neely, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mjøs, O. D.
Right arrow Articles by Neely, J. R.

Ann Thorac Surg 1991;52:965-970
© 1991 The Society of Thoracic Surgeons

Articles

Fatty acids suppress recovery of function after hypothermic perfusion

Ole D. Mjøs, MD, PhD*,a,b,c, Kazuo Ichihara, PhDa,b,c, Erik Fellenius, PhDa,b,c, Truls Myrmel, MDa,b,c, James R. Neely, PhDa,b,c

a Institute of Medical Biology, University of Tromsø, Tromsø, Norway
b Department of Pharmacology, Pharmacia AB Uppsala, Sweden
c Department of Physiology, The Milton S. Hershey Medical Center, The Pennsylvania State University Hershey, Pennsylvania USA

* Address reprint requests to Dr Mjøs, Institute of Medical Biology, University of Tromsø, N-9000 Tromsø, Norway.

Working rat hearts were perfused for 15 minutes at 37 °C before switching to a Langendorff perfusion (60 mm Hg aortic pressure) at 10 °C for 40 minutes of hypothermic arrest. Ventricular function was allowed to recover for 15 minutes at 37 °C by reestablishing the prehypothermic conditions. The perfusate was Krebs-Henseleit bicarbonate buffer containing 3% bovine serum albumin and either glucose (11 mmol/L) or glucose (11 mmol/L) plus palmitate (1.2 mmol/L) and gassed with 95% O2 and 5% CO2. In hearts receiving glucose alone as substrate, coronary flow was maintained constant during the 40 minutes of hypothermic arrest and returned to prehypothermic rates with rewarming. Ventricular function, as estimated by peak systolic pressure and heart rate, recovered to the prehypothermic level. When palmitate was added, coronary flow decreased continuously throughout the hypothermic perfusion (22% decrease by 40 minutes), and ventricular pressure development was lower throughout the rewarming perfusion. Tissue levels of adenosine triphosphate and creatine phosphate were well maintained and long-chain acyl coenzyme A and acyl carnitine decreased during hypothermia regardless of the substrate provided. With rewarming, tissue levels of adenosine triphosphate and creatine phosphate decreased in those hearts receiving palmitate. Omission of fatty acid either during hypothermia or during the first 5 minutes of rewarming improved recovery of function. Addition of oxfenicine to inhibit fatty acid oxidation, or inhibition of Ca2+ overload by verapamil and low perfusate Ca2+, prevented the effects of palmitate on ventricular function. Suppressed recovery of ventricular function after hypothermic perfusion in the presence of palmitate did not consistently correlate with low energy levels or high levels of metabolic products. Altered energy metabolism did not appear to be responsible for the fatty acid effect.




This article has been cited by other articles:


Home page
 Physiol. Rev.Home page
G. D. Lopaschuk, J. R. Ussher, C. D. L. Folmes, J. S. Jaswal, and W. C. Stanley
Myocardial Fatty Acid Metabolism in Health and Disease
Physiol Rev, January 1, 2010; 90(1): 207 - 258.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
N. F. Gilbert, P. E. Meyer, M. P. Tauriainen, R. Y. Chao, J. B. Patel, C. R. Malloy, and M. E. Jessen
Effects of hypothermia on myocardial substrate selection
Ann. Thorac. Surg., October 1, 2002; 74(4): 1208 - 1212.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
T. K. Steigen, T. Tveita, O. Hevroy, T. V. Andreasen, and T. S. Larsen
Glucose and Fatty Acid Oxidation by the In Situ Dog Heart During Experimental Cooling and Rewarming
Ann. Thorac. Surg., May 1, 1998; 65(5): 1235 - 1240.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. D. Belke, L. C. H. Wang, and G. D. Lopaschuk
Effects of hypothermia on energy metabolism in rat and Richardson's ground squirrel hearts
J Appl Physiol, April 1, 1997; 82(4): 1210 - 1218.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
T. S. Larsen, O. Irtun, T. K. Steigen, T. V. Andreasen, and D. Sorlie
Myocardial Substrate Oxidation During Warm Continuous Blood Cardioplegia
Ann. Thorac. Surg., September 1, 1996; 62(3): 762 - 768.
[Abstract] [Full Text]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
W. S. McMahon, P. C. Gillette, R. B. Hinton, J. R. Stratton, F. A. Crawford, and F. G. Spinale
DEVELOPMENTAL DIFFERENCES IN MYOCYTE CONTRACTILE RESPONSE AFTER CARDIOPLEGIC ARREST
J. Thorac. Cardiovasc. Surg., June 1, 1996; 111(6): 1257 - 1266.
[Abstract] [Full Text]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
J. R. Handy, F. G. Spinale, R. Mukherjee, and F. A. Crawford
Hypothermic potassium cardioplegia impairs myocyte recovery of contractility and inotropy
J. Thorac. Cardiovasc. Surg., April 1, 1994; 107(4): 1050 - 1058.
[Abstract] [Full Text]

Home page
 J. Thorac. Cardiovasc. Surg.Home page
T. K. Steigen, E. Aasum, T. Myrmel, and T. S. Larsen
Effects of fatty acids on myocardial calcium control during hypothermic perfusion
J. Thorac. Cardiovasc. Surg., January 1, 1994; 107(1): 233 - 241.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 1991 by The Society of Thoracic Surgeons.