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Ann Thorac Surg 1991;51:204-207
© 1991 The Society of Thoracic Surgeons

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Articles

Endothelial cell viability in the rat aortic wall

Division of Cardiothoracic Surgery, Department of Surgery, University of Iowa School of Medicine, Iowa City, Iowa USA

Accepted for publication September 27, 1990.

^_* Address reprint requests to Dr Lupinetti, Division of Cardiothoracic Surgery, University of Michigan Hospital, 2120 Taubman Center, Box 0344, Ann Arbor, MI 48109.

Aortic allografts may offer advantages over prosthetic materials for aortic valve replacement or reconstruction with a valved aortic conduit. Cellular viability may partly determine long-term allograft performance. To evaluate endothelial cell viability in a rat model, valved aortic conduits were subjected to collagenase digestion. The resulting endothelial cell suspension was labeled with Griffonia simplicifolia agglutinin-fluorescein isothiocyanate (GSA-FITC), a marker specific for vascular endothelial cells of the rat. The cells were then incubated with propidium iodide, which is excluded by viable cells. Flow cytometry evaluated endothelial cell viability by determination of percentage of GSA-FITC-positive cells that were negative for propidium iodide. Aortas were studied immediately after harvest or after storage at 4 °C in a nutrient medium for 3 to 21 days. Percentage of viable endothelial cells showed a progressive decline with increasing duration of storage. These results demonstrate flow cytometric measurement of endothelial cell viability, a factor of possible importance in assessing allograft storage methods, and show that endothelial viability declines with prolonged storage at 4 °C in a nutrient medium.

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