HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) References Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Geoffrey M. Graeber Michael J. Reardon Arthur W. Fleming Harold D. Head Rostik Zajtchuk Walter H. Brott Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Graeber, G. M. Articles by Foster, J. H. Search for Related Content PubMed PubMed Citation Articles by Graeber, G. M. Articles by Foster, J. H.

Ann Thorac Surg 1981;32:230-234
© 1981 The Society of Thoracic Surgeons

---

Articles

An Analysis of the Isoenzymes of Creatine Phosphokinase and Lactic Dehydrogenase in the Esophagus

Division of Surgery and Veterinary Pathology, Walter Reed Army Institute of Research and the Thoracic Surgery Service, Walter Reed Army Medical Center, Washington, DC, and the Department of Surgery, the Uniformed Services University of the Health Sciences, Bethesda, MD

^* Address reprint requests to Dr. Graeber, Division of Surgery, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, DC 20012

Surgical manipulation of muscular organs can cause alterations of the serum isoenzymes of creatine phosphokinase (CPK) and lactic dehydrogenase (LDH), which are frequently used to confirm the diagnosis of myocardial infarction (MI). Since the content of these enzymes and their isoenzymes has not been established for the esophagus, an experiment was conducted to evaluate and compare the enzymes in postmortem specimens from humans and fresh canine specimens. One gram transmural esophageal sections were taken from specimens having no demonstrable disease. All samples were homogenized individually in Ringer's lactate solution and centrifuged, and the supernatants were analyzed for the respective isoenzyme distributions by agarose gel electrophoresis. From the study we drew the following conclusions: (1) all three isoenzymes of CPK (including CPK-MB, the myocardial isoenzyme) are present in the esophagus; (2) LDH₁, the isoenzyme of LDH most prevalent in myocardium, is the least common of the five isoenzymes of LDH in the esophagus; (3) the dog is an appropriate model for studying changes of these isoenzymes after operation; and (4) any potential confusion in diagnosing postoperative MI due to esophageal CPK-MB in the serum can be resolved, theoretically, by analyzing LDH serum isoenzymes. In myocardial infarction, LDH, becomes the predominant isoenzyme, whereas esophageal injury should be associated, theoretically, with a serum LDH isoenzyme pattern in which LDH₁ is the least prevalent isoenzyme.

This article has been cited by other articles:

				L. G. M. Graeber, R. E. Wolf, L. R. K. H. Wong, L. D. J. Cohen, L. B. A. Grishkin, C. L. F. Johnson, and C. R. Zajtchuk Serum Creatine Kinase and Lactate Dehydrogenase Isoenzyme Levels in Patients After Major Esophageal Surgery, Esophageal Dilation, and Acute Myocardial Infarction Ann. Thorac. Surg., March 1, 1987; 43(3): 279 - 284. [Abstract] [PDF]

				M. E. Lee, D. H. Sethna, C. M. Conklin, W. E. Shell, J. M. Matloff, and R. J. Gray CK-MB Release Following Coronary Artery Bypass Grafting in the Absence of Myocardial Infarction Ann. Thorac. Surg., March 1, 1983; 35(3): 277 - 279. [Abstract] [PDF]