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Ann Thorac Surg 1976;21:230-236
© 1976 The Society of Thoracic Surgeons

Articles

Establishment of a Viable Homograft Cardiac Valve Bank: A Rapid Method of Determining Homograft Viability

Lesley K. Watts, B.Sc., Patrick Duffy, M.B., Ph.D., Ruth B. Field, S.R.N., E.G. Stafford, F.R.A.C.S., Mark F. O'Brien, F.R.A.C.S.*

From the Departments of Biochemistry and Cardiac Surgery, The Prince Charles Hospital, Chermside, Queensland, Australia

Accepted for publication July 9, 1975.

* Address reprint requests to Dr. O'Brien, Department of Cardiac Surgery, The Prince Charles Hospital, Chermside, Queensland, Australia

A method for determining the viability of homograft valves has been developed based on sequential measurements of glucose and pH levels of the culture medium in which cardiac valves have been maintained for short periods at 37°. Viable valves, as determined by tissue culture, showed a characteristic pattern of glucose utilization and pH reduction that was absent in nonviable valves. Upon explantation of valve leaflet fragments into tissue culture, only fragments from valves that metabolized glucose produced viable fibroblast cultures.

The method reported here is rapid, requires no specialized equipment, is nondestructive, and can directly determine the viability of the valve homo-graft within 24 to 48 hours.




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